Ser126
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Home > Phosphorylation Site Page: > Ser126  -  IGFBP1 (human)

Site Information
GsPEsPEstEITEEE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451658

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 10 ) , [32P] bio-synthetic labeling ( 16 , 17 ) , electrophoretic mobility shift ( 15 , 16 ) , immunoprecipitation ( 1 , 2 , 12 , 17 ) , mass spectrometry ( 1 , 2 , 5 , 6 , 7 , 11 , 12 , 13 , 14 , 15 ) , multiple reaction monitoring (MRM) ( 3 ) , mutation of modification site ( 10 , 16 , 17 ) , peptide sequencing ( 16 , 17 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ) , phosphoamino acid analysis ( 16 ) , western blotting ( 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 )
Disease tissue studied:
liver cancer ( 2 , 6 , 7 , 8 , 9 , 15 ) , Fetal growth restriction ( 11 , 14 )
Relevant cell line - cell type - tissue:
amniotic fluid ( 11 , 12 , 13 , 14 ) , blood ( 9 ) , CHO (fibroblast) [EphB1 (human), transfection] ( 16 , 17 ) , CHO (fibroblast) ( 10 ) , endometrial stromal ( 1 , 3 ) , HepG2 (hepatic) ( 2 , 6 , 7 , 8 , 9 , 15 ) , stromal ( 5 )

Upstream Regulation
Regulatory protein:
DEPTOR (human) ( 8 ) , ERK1 (human) ( 8 ) , GCN2 (human) ( 8 ) , Raptor (human) ( 7 , 8 , 9 ) , RICTOR (human) ( 7 , 8 , 9 ) , TSC2 (human) ( 7 )
Putative in vivo kinases:
CK2A1 (human) ( 6 , 8 , 9 ) , PKACA (human) ( 1 ) , PKCA (human) ( 2 , 6 )
Kinases, in vitro:
CK2A1 (human) ( 6 )
Treatments:
8-Rp-cAMP ( 5 ) , amino_acid_starvation ( 15 ) , bisindolylmaleimide_II ( 2 ) , fetal growth restriction ( 9 ) , hypoxia ( 1 , 3 , 7 , 15 ) , leucine ( 8 ) , leucine_deprivation ( 2 , 5 , 6 , 8 ) , MPA ( 5 ) , phorbol_ester ( 2 ) , rapamycin ( 3 , 7 , 8 , 9 ) , siRNA ( 2 ) , TBB ( 2 , 8 ) , U0126 ( 8 )

Downstream Regulation
Effects of modification on IGFBP1:
activity, induced ( 15 ) , activity, inhibited ( 3 , 8 ) , molecular association, regulation ( 3 , 11 , 15 ) , phosphorylation ( 8 , 10 )
Effects of modification on biological processes:
signaling pathway regulation ( 3 )
Induce interaction with:
CK2B (human) ( 3 ) , IGF1 (human) ( 11 , 15 , 16 )

Disease / Diagnostics Relevance
Relevant diseases:
Fetal growth restriction ( 8 , 11 )

References 

1

Gupta MB, et al. (2022) Increased Colocalization and Interaction Between Decidual Protein Kinase A and Insulin-like Growth Factor-Binding Protein-1 in Intrauterine Growth Restriction. J Histochem Cytochem 70, 515-530
35801847   Curated Info

2

Chen AW, et al. (2021) IGFBP-1 hyperphosphorylation in response to nutrient deprivation is mediated by activation of protein kinase Cα (PKCα). Mol Cell Endocrinol 536, 111400
34314739   Curated Info

3

Abu Shehab M, et al. (2020) Inhibition of decidual IGF-1 signaling in response to hypoxia and leucine deprivation is mediated by mTOR and AAR pathways and increased IGFBP-1 phosphorylation. Mol Cell Endocrinol 512, 110865
32502935   Curated Info

4

Singal SS, et al. (2018) Increased Insulin-like Growth Factor Binding Protein-1 Phosphorylation in Decidualized Stromal Mesenchymal Cells in Human Intrauterine Growth Restriction Placentas. J Histochem Cytochem, 22155418772574
29718759   Curated Info

5

Shehab MA, et al. (2017) Exposure of decidualized HIESC to low oxygen tension and leucine deprivation results in increased IGFBP-1 phosphorylation and reduced IGF-I bioactivity. Mol Cell Endocrinol 452, 1-14
28435049   Curated Info

6

Malkani N, et al. (2016) Increased IGFBP-1 phosphorylation in response to leucine deprivation is mediated by CK2 and PKC. Mol Cell Endocrinol 425, 48-60
26733150   Curated Info

7

Damerill I, et al. (2016) Hypoxia Increases IGFBP-1 Phosphorylation Mediated by mTOR Inhibition. Mol Endocrinol 30, 201-16
26714229   Curated Info

8

Malkani N, Jansson T, Gupta MB (2015) IGFBP-1 hyperphosphorylation in response to leucine deprivation is mediated by the AAR pathway. Mol Cell Endocrinol 412, 182-95
25957086   Curated Info

9

Abu Shehab M, et al. (2014) Liver mTOR controls IGF-I bioavailability by regulation of protein kinase CK2 and IGFBP-1 phosphorylation in fetal growth restriction. Endocrinology 155, 1327-39
24437487   Curated Info

10

Abu Shehab M, et al. (2013) Phosphorylation of IGFBP-1 at Discrete Sites Elicits Variable Effects on IGF-I Receptor Autophosphorylation. Endocrinology 154, 1130-43
23354097   Curated Info

11

Abu Shehab M, et al. (2010) Site-specific IGFBP-1 hyper-phosphorylation in fetal growth restriction: clinical and functional relevance. J Proteome Res 9, 1873-81
20143870   Curated Info

12

Abu Shehab M, Inoue S, Han VK, Gupta MB (2009) Site specific phosphorylation of insulin-like growth factor binding protein-1 (IGFBP-1) for evaluating clinical relevancy in fetal growth restriction. J Proteome Res 8, 5325-35
19731965   Curated Info

13

Dolcini L, et al. (2009) Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms. FEBS J 276, 6033-46
19765076   Curated Info

14

Nissum M, et al. (2009) Functional and complementary phosphorylation state attributes of human insulin-like growth factor-binding protein-1 (IGFBP-1) isoforms resolved by free flow electrophoresis. Mol Cell Proteomics 8, 1424-35
19193607   Curated Info

15

Seferovic MD, et al. (2009) Hypoxia and leucine deprivation induce human insulin-like growth factor binding protein-1 hyperphosphorylation and increase its biological activity. Endocrinology 150, 220-31
18772238   Curated Info

16

Jones JI, Busby WH, Wright G, Clemmons DR (1993) Human IGFBP-1 is phosphorylated on 3 serine residues: effects of site-directed mutagenesis of the major phosphoserine. Growth Regul 3, 37-40
7683525   Curated Info

17

Jones JI, et al. (1993) Identification of the sites of phosphorylation in insulin-like growth factor binding protein-1. Regulation of its affinity by phosphorylation of serine 101. J Biol Chem 268, 1125-31
7678248   Curated Info