Ser15
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Home > Phosphorylation Site Page: > Ser15  -  TTK (human)

Site Information
GRELtIDsIMNkVRD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 13194900

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 6 ) , [32P] bio-synthetic labeling ( 6 ) , immunoassay ( 5 ) , immunoprecipitation ( 6 ) , mass spectrometry ( 4 , 7 ) , mass spectrometry (in vitro) ( 6 ) , mutation of modification site ( 1 , 3 , 5 , 6 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Disease tissue studied:
breast cancer ( 2 ) , colorectal cancer ( 2 , 5 , 6 ) , colorectal carcinoma ( 2 , 5 , 6 ) , lung cancer ( 4 ) , non-small cell lung cancer ( 4 ) , non-small cell lung adenocarcinoma ( 4 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 4 ) , HCT116 (intestinal) ( 2 ) , HEK293T (epithelial) ( 6 ) , HeLa (cervical) ( 1 , 3 ) , Jurkat (T lymphocyte) ( 7 ) , MDA-MB-231 (breast cell) ( 2 ) , SW480 (intestinal) ( 5 , 6 )

Upstream Regulation
Putative in vivo kinases:
TTK (human) ( 1 )
Kinases, in vitro:
TTK (human) ( 6 )
Treatments:
CFI-402257 ( 2 ) , nocodazole ( 6 )

Downstream Regulation
Effects of modification on TTK:
intracellular localization ( 1 , 6 )

References 

1

Hayward D, Roberts E, Gruneberg U (2022) MPS1 localizes to end-on microtubule-attached kinetochores to promote microtubule release. Curr Biol 32, 5200-5208.e8
36395767   Curated Info

2

Mason JM, et al. (2017) Functional characterization of CFI-402257, a potent and selective Mps1/TTK kinase inhibitor, for the treatment of cancer. Proc Natl Acad Sci U S A 114, 3127-3132
28270606   Curated Info

3

Wang X, et al. (2014) Dynamic autophosphorylation of mps1 kinase is required for faithful mitotic progression. PLoS One 9, e104723
25265012   Curated Info

4

Rikova K (2012) CST Curation Set: 13324; Year: 2012; Biosample/Treatment: cell line, A549/untreated; Disease: lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Zhang X, et al. (2011) Two LXXLL motifs in the N terminus of Mps1 are required for Mps1 nuclear import during G(2)/M transition and sustained spindle checkpoint responses. Cell Cycle 10, 2742-50
21778823   Curated Info

6

Xu Q, et al. (2009) Regulation of kinetochore recruitment of two essential mitotic spindle checkpoint proteins by Mps1 phosphorylation. Mol Biol Cell 20, 10-20
18923149   Curated Info

7

Possemato A (2008) CST Curation Set: 3661; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS:pSXXXpS
Curated Info

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