Tyr246
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Home > Phosphorylation Site Page: > Tyr246  -  RACK1 (human)

Site Information
LCFSPNryWLCAATG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451481
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 , 6 ) , mass spectrometry ( 5 , 8 ) , mutation of modification site ( 2 , 3 , 4 , 6 , 7 , 9 ) , phospho-antibody ( 4 , 9 ) , western blotting ( 4 , 6 , 9 )
Disease tissue studied:
colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , lymphoma ( 5 , 8 ) , Hodgkin's lymphoma ( 5 , 8 ) , neuroblastoma ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Src (human) ( 4 , 9 )
Kinases, in vitro:
Src (human) ( 9 )
Treatments:
PP2 ( 4 )

Downstream Regulation
Effects of modification on RACK1:
activity, inhibited ( 6 ) , intracellular localization ( 3 , 4 ) , molecular association, regulation ( 4 , 6 , 9 ) , phosphorylation ( 6 ) , protein stabilization ( 3 ) , ubiquitination ( 3 )
Effects of modification on biological processes:
carcinogenesis, inhibited ( 3 ) , cell adhesion, induced ( 3 ) , cell motility, altered ( 6 ) , cell motility, inhibited ( 3 ) , endocytosis, inhibited ( 3 ) , transcription, inhibited ( 7 ) , translation, altered ( 4 )
Induce interaction with:
Src (human) ( 6 ) , ZBP1 (human) ( 4 )

References 

1

Ullah H, Hou W, Dakshanamurthy S, Tang Q (2019) Host targeted antiviral (HTA): functional inhibitor compounds of scaffold protein RACK1 inhibit herpes simplex virus proliferation. Oncotarget 10, 3209-3226
31143369   Curated Info

2

Yang J, et al. (2012) Receptor for Activated C Kinase 1 (RACK1) Inhibits Function of Transient Receptor Potential (TRP)-type Channel Pkd2L1 through Physical Interaction. J Biol Chem 287, 6551-61
22174419   Curated Info

3

Swaminathan G, Cartwright CA (2012) Rack1 promotes epithelial cell-cell adhesion by regulating E-cadherin endocytosis. Oncogene 31, 376-89
21685945   Curated Info

4

Ceci M, et al. (2012) RACK1 Is a Ribosome Scaffold Protein for β-actin mRNA/ZBP1 Complex. PLoS One 7, e35034
22523568   Curated Info

5

Gu TL, et al. (2010) Identification of activated Tnk1 kinase in Hodgkin's lymphoma. Leukemia 24, 861-5
20090780   Curated Info

6

Doan AT, Huttenlocher A (2007) RACK1 regulates Src activity and modulates paxillin dynamics during cell migration. Exp Cell Res 313, 2667-79
17574549   Curated Info

7

Okano K, Schnaper HW, Bomsztyk K, Hayashida T (2006) RACK1 binds to Smad3 to modulate transforming growth factor-beta1-stimulated alpha2(I) collagen transcription in renal tubular epithelial cells. J Biol Chem 281, 26196-204
16849317   Curated Info

8

Gu T (2006) CST Curation Set: 1627; Year: 2006; Biosample/Treatment: cell line, L540/untreated; Disease: Hodgkin's lymphoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Chang BY, Harte RA, Cartwright CA (2002) RACK1: a novel substrate for the Src protein-tyrosine kinase. Oncogene 21, 7619-29
12400005   Curated Info