Ser88
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Home > Phosphorylation Site Page: > Ser88  -  PLM (rat)

Site Information
RSsIRRLstRRR___   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451425

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 11 ) , back-titration ( 9 ) , immunoprecipitation ( 1 , 2 , 4 , 7 ) , mass spectrometry ( 4 ) , mutation of modification site ( 4 , 5 , 10 ) , phospho-antibody ( 1 , 3 , 6 , 7 , 9 ) , phosphoamino acid analysis ( 11 ) , western blotting ( 1 , 3 , 4 , 5 , 6 , 7 , 9 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 4 ) , 293 (epithelial) ( 1 , 10 ) , 293FT ( 2 ) , brain ( 4 ) , cardiac-heart ( 1 , 2 ) , heart ( 6 ) , HeLa (cervical) ( 9 ) , myocyte-heart ( 2 , 3 , 4 , 6 , 7 ) , oocyte ( 5 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 7 ) , PKCA (human) ( 7 )
Kinases, in vitro:
PKACA (human) ( 8 , 9 ) , PKACA (rat) ( 11 ) , PKCA (rat) ( 11 )
Putative upstream phosphatases:
PPP1CA (rat) ( 1 )
Treatments:
bisindolylmaleimide ( 4 , 7 ) , colforsin ( 7 ) , endothelin ( 7 ) , formaldehyde ( 4 ) , H-89 ( 7 ) , inhibitor-1 ( 6 ) , insulin ( 11 ) , isoproterenol ( 4 , 6 , 7 ) , NKH_477 ( 4 ) , okadaic_acid ( 6 ) , phenylephrine ( 4 , 7 ) , phorbol_ester ( 4 , 7 ) , racepinefrine ( 11 )

Downstream Regulation
Effects of modification on PLM:
activity, induced ( 6 , 7 , 8 ) , activity, inhibited ( 10 ) , molecular association, regulation ( 2 )
Induce interaction with:
ZDHHC5 (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
cardiomyopathy ( 6 )

References 

1

Hafver TL, et al. (2016) Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman. J Biol Chem 291, 4561-79
26668322   Curated Info

2

Howie J, et al. (2014) Substrate recognition by the cell surface palmitoyl transferase DHHC5. Proc Natl Acad Sci U S A 111, 17534-9
25422474   Curated Info

3

Pavlovic D, et al. (2013) Nitric oxide regulates cardiac intracellular Na(+) and Ca(2+) by modulating Na/K ATPase via PKCε and phospholemman-dependent mechanism. J Mol Cell Cardiol 61, 164-71
23612119   Curated Info

4

Wypijewski KJ, et al. (2013) A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle. J Biol Chem 288, 13808-20
23532852   Curated Info

5

Moshitzky S, Asher C, Garty H (2012) Intracellular Trafficking of FXYD1 (Phospholemman) and FXYD7 Proteins in Xenopus Oocytes and Mammalian Cells. J Biol Chem 287, 21130-41
22535957   Curated Info

6

El-Armouche A, et al. (2011) Phospholemman-dependent regulation of the cardiac Na/K-ATPase activity is modulated by inhibitor-1 sensitive type-1 phosphatase. FASEB J 25, 4467-75
21849407   Curated Info

7

Fuller W, et al. (2009) FXYD1 phosphorylation in vitro and in adult rat cardiac myocytes: threonine 69 is a novel substrate for protein kinase C. Am J Physiol Cell Physiol 296, C1346-55
19339511   Curated Info

8

Pavlović D, Fuller W, Shattock MJ (2007) The intracellular region of FXYD1 is sufficient to regulate cardiac Na/K ATPase. FASEB J 21, 1539-46
17283221   Curated Info

9

Lifshitz Y, Lindzen M, Garty H, Karlish SJ (2006) Functional interactions of phospholemman (PLM) (FXYD1) with Na+,K+-ATPase. Purification of alpha1/beta1/PLM complexes expressed in Pichia pastoris. J Biol Chem 281, 15790-9
16608841   Curated Info

10

Zhang XQ, et al. (2006) Phospholemman inhibition of the cardiac Na+/Ca2+ exchanger. Role of phosphorylation. J Biol Chem 281, 7784-92
16434394   Curated Info

11

Walaas SI, et al. (1994) Protein kinase C and cyclic AMP-dependent protein kinase phosphorylate phospholemman, an insulin and adrenaline-regulated membrane phosphoprotein, at specific sites in the carboxy terminal domain. Biochem J 304 ( Pt 2), 635-40
7999001   Curated Info