Ser83
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Home > Phosphorylation Site Page: > Ser83  -  PLM (rat)

Site Information
EEGtFRSsIRRLstR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451424

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 8 ) , immunoprecipitation ( 1 , 3 , 6 ) , mass spectrometry ( 3 ) , mutation of modification site ( 3 , 4 , 7 ) , phospho-antibody ( 2 , 5 , 6 ) , phosphoamino acid analysis ( 8 ) , western blotting ( 2 , 3 , 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 3 ) , 293 (epithelial) ( 7 ) , 293FT ( 1 ) , brain ( 3 ) , cardiac-heart ( 1 ) , heart ( 5 ) , myocyte-heart ( 1 , 2 , 3 , 5 , 6 ) , oocyte ( 4 )

Upstream Regulation
Regulatory protein:
PKCA (human) ( 3 )
Putative in vivo kinases:
PKACA (human) ( 6 ) , PKCA (human) ( 6 )
Kinases, in vitro:
PKCA (rat) ( 8 )
Putative upstream phosphatases:
PPP2CA (human) ( 3 )
Treatments:
bisindolylmaleimide ( 3 , 6 ) , colforsin ( 6 ) , endothelin ( 6 ) , formaldehyde ( 3 ) , H-89 ( 6 ) , inhibitor-1 ( 5 ) , insulin ( 8 ) , isoproterenol ( 3 , 5 , 6 ) , NKH_477 ( 3 ) , okadaic_acid ( 5 ) , phenylephrine ( 3 , 6 ) , phorbol_ester ( 3 , 6 )

Downstream Regulation
Effects of modification on PLM:
activity, induced ( 6 , 7 ) , intracellular localization ( 3 ) , molecular association, regulation ( 1 , 3 )
Induce interaction with:
ZDHHC5 (human) ( 1 )
Inhibit interaction with:
caveolin-3 (human) ( 3 )

References 

1

Howie J, et al. (2014) Substrate recognition by the cell surface palmitoyl transferase DHHC5. Proc Natl Acad Sci U S A 111, 17534-9
25422474   Curated Info

2

Pavlovic D, et al. (2013) Nitric oxide regulates cardiac intracellular Na(+) and Ca(2+) by modulating Na/K ATPase via PKCĪµ and phospholemman-dependent mechanism. J Mol Cell Cardiol 61, 164-71
23612119   Curated Info

3

Wypijewski KJ, et al. (2013) A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle. J Biol Chem 288, 13808-20
23532852   Curated Info

4

Moshitzky S, Asher C, Garty H (2012) Intracellular Trafficking of FXYD1 (Phospholemman) and FXYD7 Proteins in Xenopus Oocytes and Mammalian Cells. J Biol Chem 287, 21130-41
22535957   Curated Info

5

El-Armouche A, et al. (2011) Phospholemman-dependent regulation of the cardiac Na/K-ATPase activity is modulated by inhibitor-1 sensitive type-1 phosphatase. FASEB J 25, 4467-75
21849407   Curated Info

6

Fuller W, et al. (2009) FXYD1 phosphorylation in vitro and in adult rat cardiac myocytes: threonine 69 is a novel substrate for protein kinase C. Am J Physiol Cell Physiol 296, C1346-55
19339511   Curated Info

7

Zhang XQ, et al. (2006) Phospholemman inhibition of the cardiac Na+/Ca2+ exchanger. Role of phosphorylation. J Biol Chem 281, 7784-92
16434394   Curated Info

8

Walaas SI, et al. (1994) Protein kinase C and cyclic AMP-dependent protein kinase phosphorylate phospholemman, an insulin and adrenaline-regulated membrane phosphoprotein, at specific sites in the carboxy terminal domain. Biochem J 304 ( Pt 2), 635-40
7999001   Curated Info