Thr81
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Home > Phosphorylation Site Page: > Thr81  -  p53 (human)

Site Information
APAPAAPtPAAPAPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447540

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 3 , 10 ) , mutation of modification site ( 1 , 2 , 5 , 7 , 8 , 10 ) , phospho-antibody ( 1 , 4 , 6 , 7 , 9 , 10 ) , western blotting ( 1 , 4 , 9 )
Disease tissue studied:
bladder cancer ( 1 ) , bone cancer ( 5 ) , colorectal cancer ( 5 ) , colorectal carcinoma ( 5 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 ) , neuroblastoma ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
JNK1 (human) ( 6 , 10 )
Kinases, in vitro:
JNK1 (human) ( 10 )
Treatments:
15d-PGJ2 ( 4 ) , LLnL ( 7 ) , SB203580 ( 4 ) , SP600125 ( 1 , 4 ) , UV ( 6 , 7 , 9 , 10 ) , WR1065 ( 6 )

Downstream Regulation
Effects of modification on p53:
acetylation ( 5 ) , molecular association, regulation ( 5 , 7 , 8 ) , protein stabilization ( 1 , 10 )
Effects of modification on biological processes:
apoptosis, induced ( 1 , 5 , 10 ) , transcription, altered ( 8 ) , transcription, induced ( 5 , 10 )
Induce interaction with:
DNA ( 5 ) , PIN1 (human) ( 5 , 7 , 8 )
Inhibit interaction with:
MDM2 (human) ( 1 ) , PPP1R13L (human) ( 5 )

References 

1

Pan CW, et al. (2016) JNK2 downregulation promotes tumorigenesis and chemoresistance by decreasing p53 stability in bladder cancer. Oncotarget 7, 35119-31
27147566   Curated Info

2

Grison A, et al. (2011) Ser46 phosphorylation and prolyl-isomerase Pin1-mediated isomerization of p53 are key events in p53-dependent apoptosis induced by mutant huntingtin. Proc Natl Acad Sci U S A 108, 17979-84
22011578   Curated Info

3

Possemato A (2009) CST Curation Set: 6443; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Ho TC, et al. (2008) 15-deoxy-Delta(12,14)-prostaglandin J2 induces vascular endothelial cell apoptosis through the sequential activation of MAPKS and p53. J Biol Chem 283, 30273-88
18718914   Curated Info

5

Mantovani F, et al. (2007) The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat Struct Mol Biol 14, 912-20
17906639   Curated Info

6

Pluquet O, et al. (2003) The cytoprotective aminothiol WR1065 activates p53 through a non-genotoxic signaling pathway involving c-Jun N-terminal kinase. J Biol Chem 278, 11879-87
12531896   Curated Info

7

Zheng H, et al. (2002) The prolyl isomerase Pin1 is a regulator of p53 in genotoxic response. Nature 419, 849-53
12397361   Curated Info

8

Zacchi P, et al. (2002) The prolyl isomerase Pin1 reveals a mechanism to control p53 functions after genotoxic insults. Nature 419, 853-7
12397362   Curated Info

9

Minamoto T, et al. (2001) Distinct pattern of p53 phosphorylation in human tumors. Oncogene 20, 3341-7
11423984   Curated Info

10

Buschmann T, et al. (2001) Jun NH2-terminal kinase phosphorylation of p53 on Thr-81 is important for p53 stabilization and transcriptional activities in response to stress. Mol Cell Biol 21, 2743-54
11283254   Curated Info