Thr81
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Home > Phosphorylation Site Page: > Thr81  -  p53 (human)

Site Information
APAPAAPtPAAPAPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447540

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 5 , 12 ) , mutation of modification site ( 3 , 4 , 7 , 9 , 10 , 12 ) , phospho-antibody ( 1 , 2 , 3 , 6 , 8 , 9 , 11 , 12 ) , western blotting ( 1 , 2 , 3 , 6 , 11 )
Disease tissue studied:
bladder cancer ( 3 ) , bone cancer ( 1 , 7 ) , colorectal cancer ( 7 ) , colorectal carcinoma ( 7 ) , lung cancer ( 7 ) , non-small cell lung cancer ( 7 ) , neuroblastoma ( 4 )
Relevant cell line - cell type - tissue:
A2182 ( 11 ) , Aspc1 (pancreatic) ( 11 ) , COLO-320 (intestinal) ( 11 ) , HCT116 (intestinal) ( 7 ) , HEK293T (epithelial) ( 12 ) , HT116 ( 11 ) , HUVEC (endothelial) ( 6 ) , IMR-90 (fibroblast) ( 2 ) , Jurkat (T lymphocyte) ( 5 ) , MCF-7 (breast cell) ( 8 ) , NCI-H1299 (pulmonary) ( 7 , 12 ) , NCI-H596 (pulmonary) ( 11 ) , NHF (fibroblast) ( 12 ) , OM431 ( 11 ) , Saos-2 (bone cell) ( 7 , 10 ) , SH-SY5Y (neural crest) ( 4 ) , SJSA-1 (bone cell) ( 11 ) , SW680 (intestinal) ( 11 ) , T24 (bladder cell) ( 3 ) , U2OS (bone cell) [GR (human)] ( 9 ) , U2OS (bone cell) ( 1 ) , WM4 ( 11 ) , WM5 ( 11 ) , WM793 ( 11 )

Upstream Regulation
Regulatory protein:
CDC25B (human) ( 2 ) , IFI16 (human) ( 1 )
Putative in vivo kinases:
JNK1 (human) ( 8 , 12 )
Kinases, in vitro:
JNK1 (human) ( 12 )
Treatments:
15d-PGJ2 ( 6 ) , LLnL ( 9 ) , nutlin-3 ( 1 ) , SB203580 ( 6 ) , SP600125 ( 3 , 6 ) , UV ( 8 , 9 , 11 , 12 ) , WR1065 ( 8 )

Downstream Regulation
Effects of modification on p53:
acetylation ( 7 ) , molecular association, regulation ( 7 , 9 , 10 ) , protein degradation ( 2 ) , protein stabilization ( 3 , 12 )
Effects of modification on biological processes:
apoptosis, induced ( 3 , 7 , 12 ) , carcinogenesis, induced ( 2 ) , cell cycle regulation ( 2 ) , cell growth, induced ( 2 ) , transcription, altered ( 10 ) , transcription, induced ( 7 , 12 )
Induce interaction with:
DNA ( 7 ) , PIN1 (human) ( 7 , 9 , 10 )
Inhibit interaction with:
MDM2 (human) ( 3 ) , PPP1R13L (human) ( 7 )

References 

1

Li D, et al. (2021) STING-mediated degradation of IFI16 negatively regulates apoptosis by inhibiting p53 phosphorylation at serine 392. J Biol Chem 297, 100930
34216619   Curated Info

2

Chen YC, et al. (2021) CDC25B induces cellular senescence and correlates with tumor suppression in a p53-dependent manner. J Biol Chem 296, 100564
33745968   Curated Info

3

Pan CW, et al. (2016) JNK2 downregulation promotes tumorigenesis and chemoresistance by decreasing p53 stability in bladder cancer. Oncotarget 7, 35119-31
27147566   Curated Info

4

Grison A, et al. (2011) Ser46 phosphorylation and prolyl-isomerase Pin1-mediated isomerization of p53 are key events in p53-dependent apoptosis induced by mutant huntingtin. Proc Natl Acad Sci U S A 108, 17979-84
22011578   Curated Info

5

Possemato A (2009) CST Curation Set: 6443; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Ho TC, et al. (2008) 15-deoxy-Delta(12,14)-prostaglandin J2 induces vascular endothelial cell apoptosis through the sequential activation of MAPKS and p53. J Biol Chem 283, 30273-88
18718914   Curated Info

7

Mantovani F, et al. (2007) The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat Struct Mol Biol 14, 912-20
17906639   Curated Info

8

Pluquet O, et al. (2003) The cytoprotective aminothiol WR1065 activates p53 through a non-genotoxic signaling pathway involving c-Jun N-terminal kinase. J Biol Chem 278, 11879-87
12531896   Curated Info

9

Zheng H, et al. (2002) The prolyl isomerase Pin1 is a regulator of p53 in genotoxic response. Nature 419, 849-53
12397361   Curated Info

10

Zacchi P, et al. (2002) The prolyl isomerase Pin1 reveals a mechanism to control p53 functions after genotoxic insults. Nature 419, 853-7
12397362   Curated Info

11

Minamoto T, et al. (2001) Distinct pattern of p53 phosphorylation in human tumors. Oncogene 20, 3341-7
11423984   Curated Info

12

Buschmann T, et al. (2001) Jun NH2-terminal kinase phosphorylation of p53 on Thr-81 is important for p53 stabilization and transcriptional activities in response to stress. Mol Cell Biol 21, 2743-54
11283254   Curated Info

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