Ser6
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser6  -  p53 (human)

Site Information
__MEEPQsDPsVEPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447537

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 17 ) , immunoprecipitation ( 1 , 19 , 25 ) , mass spectrometry ( 2 , 4 , 6 ) , modification-specific antibody ( 19 , 20 ) , mutation of modification site ( 1 , 3 , 18 , 20 , 23 , 27 ) , phospho-antibody ( 1 , 3 , 5 , 7 , 9 , 10 , 12 , 13 , 14 , 15 , 16 , 17 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 28 , 29 ) , western blotting ( 1 , 3 , 5 , 7 , 10 , 12 , 15 , 17 , 19 , 20 , 21 , 23 , 28 , 29 )
Disease tissue studied:
ataxia-telangiectasia ( 15 , 25 ) , bladder cancer ( 1 ) , bone cancer ( 3 , 17 ) , breast cancer ( 10 ) , colorectal cancer ( 12 , 18 , 19 , 20 ) , colorectal carcinoma ( 12 , 18 , 19 , 20 ) , leukemia ( 7 ) , acute lymphocytic leukemia ( 7 ) , lung cancer ( 3 , 4 , 9 , 10 , 12 , 20 , 21 , 22 , 29 ) , non-small cell lung cancer ( 3 , 4 , 9 , 10 , 12 , 20 ) , non-small cell lung adenocarcinoma ( 4 ) , non-small cell squamous cell lung carcinoma ( 12 ) , neuroblastoma ( 25 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
LKB1 (human) ( 12 )
Treatments:
2-deoxyglucose ( 9 ) , adriamycin ( 7 , 15 , 16 , 17 , 20 , 25 , 29 ) , asbestos ( 21 ) , colforsin ( 7 ) , DDATHF ( 19 ) , dexamethasone ( 22 ) , etoposide ( 16 , 19 ) , heat_shock ( 26 ) , IBMX ( 7 ) , ionizing_radiation ( 19 , 20 , 25 , 26 , 29 ) , nocodazole ( 20 ) , PALA ( 20 ) , PD98059 ( 10 ) , silibinin ( 14 ) , siRNA ( 12 ) , SNP ( 23 ) , SP600125 ( 1 ) , taxol ( 20 ) , UV ( 14 , 20 , 24 , 28 , 29 )

Downstream Regulation
Effects of modification on p53:
acetylation ( 20 , 25 ) , activity, induced ( 16 ) , phosphorylation ( 20 )
Effects of modification on biological processes:
apoptosis, altered ( 12 , 16 ) , apoptosis, induced ( 18 ) , transcription, altered ( 18 ) , transcription, induced ( 3 )

References 

1

Pan CW, et al. (2016) JNK2 downregulation promotes tumorigenesis and chemoresistance by decreasing p53 stability in bladder cancer. Oncotarget 7, 35119-31
27147566   Curated Info

2

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

3

Ozeki C, et al. (2011) Cancer susceptibility polymorphism of p53 at codon 72 affects phosphorylation and degradation of p53 protein. J Biol Chem 286, 18251-60
21454683   Curated Info

4

Rikova K (2011) CST Curation Set: 10883; Year: 2011; Biosample/Treatment: cell line, NCI-H838/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

5

Park J, et al. (2010) Dyrk1A phosphorylates p53 and inhibits proliferation of embryonic neuronal cells. J Biol Chem 285, 31895-906
20696760   Curated Info

6

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

7

Safa M, et al. (2010) Inhibitory role of cAMP on doxorubicin-induced apoptosis in pre-B ALL cells through dephosphorylation of p53 serine residues. Apoptosis 15, 196-203
19882354   Curated Info

8

Nishimura T, et al. (2009) Hepatitis C virus impairs p53 via persistent overexpression of 3beta-hydroxysterol Delta24-reductase. J Biol Chem 284, 36442-52
19861417   Curated Info

9

Zhong D, et al. (2009) The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathways through IGF1R. J Biol Chem 284, 23225-33
19574224   Curated Info

10

Adorno M, et al. (2009) A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell 137, 87-98
19345189   Curated Info

11

Warnock LJ, et al. (2008) Influence of tetramerisation on site-specific post-translational modifications of p53: comparison of human and murine p53 tumor suppressor protein. Cancer Biol Ther 7, 1481-9
18769132   Curated Info

12

Zhong D, et al. (2008) LKB1 is necessary for Akt-mediated phosphorylation of proapoptotic proteins. Cancer Res 68, 7270-7
18794113   Curated Info

13

Mayo LD, et al. (2005) Phosphorylation of human p53 at serine 46 determines promoter selection and whether apoptosis is attenuated or amplified. J Biol Chem 280, 25953-9
15843377   Curated Info

14

Dhanalakshmi S, Agarwal C, Singh RP, Agarwal R (2005) Silibinin up-regulates DNA-protein kinase-dependent p53 activation to enhance UVB-induced apoptosis in mouse epithelial JB6 cells. J Biol Chem 280, 20375-83
15792956   Curated Info

15

Kurz EU, Douglas P, Lees-Miller SP (2004) Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species. J Biol Chem 279, 53272-81
15489221   Curated Info

16

Thompson T, et al. (2004) Phosphorylation of p53 on key serines is dispensable for transcriptional activation and apoptosis. J Biol Chem 279, 53015-22
15471885   Curated Info

17

Jackson MW, et al. (2004) Limited role of N-terminal phosphoserine residues in the activation of transcription by p53. Oncogene 23, 4477-87
15064747   Curated Info

18

Kaeser MD, Pebernard S, Iggo RD (2004) Regulation of p53 stability and function in HCT116 colon cancer cells. J Biol Chem 279, 7598-605
14665630   Curated Info

19

Bronder JL, Moran RG (2003) A defect in the p53 response pathway induced by de novo purine synthesis inhibition. J Biol Chem 278, 48861-71
14517211   Curated Info

20

Saito S, et al. (2003) Phosphorylation site interdependence of human p53 post-translational modifications in response to stress. J Biol Chem 278, 37536-44
12860987   Curated Info

21

Matsuoka M, Igisu H, Morimoto Y (2003) Phosphorylation of p53 protein in A549 human pulmonary epithelial cells exposed to asbestos fibers. Environ Health Perspect 111, 509-12
12676607   Curated Info

22

Urban G, et al. (2003) Identification of a functional link for the p53 tumor suppressor protein in dexamethasone-induced growth suppression. J Biol Chem 278, 9747-53
12519780   Curated Info

23

Kim SJ, et al. (2002) p38 kinase regulates nitric oxide-induced apoptosis of articular chondrocytes by accumulating p53 via NFkappa B-dependent transcription and stabilization by serine 15 phosphorylation. J Biol Chem 277, 33501-8
12091386   Curated Info

24

Bulavin DV, et al. (2002) Amplification of PPM1D in human tumors abrogates p53 tumor-suppressor activity. Nat Genet 31, 210-5
12021785   Curated Info

25

Saito S, et al. (2002) ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation. J Biol Chem 277, 12491-4
11875057   Curated Info

26

Miyakoda M, Suzuki K, Kodama S, Watanabe M (2002) Activation of ATM and phosphorylation of p53 by heat shock. Oncogene 21, 1090-6
11850826   Curated Info

27

Dumaz N, Milne DM, Jardine LJ, Meek DW (2001) Critical roles for the serine 20, but not the serine 15, phosphorylation site and for the polyproline domain in regulating p53 turnover. Biochem J 359, 459-64
11583595   Curated Info

28

Minamoto T, et al. (2001) Distinct pattern of p53 phosphorylation in human tumors. Oncogene 20, 3341-7
11423984   Curated Info

29

Higashimoto Y, et al. (2000) Human p53 is phosphorylated on serines 6 and 9 in response to DNA damage-inducing agents. J Biol Chem 275, 23199-203
10930428   Curated Info