Ser378
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Home > Phosphorylation Site Page: > Ser378  -  p53 (human)

Site Information
skkGQstsRHkkLMF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447534

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 9 ) , mass spectrometry (in vitro) ( 3 , 9 ) , mutation of modification site ( 4 , 5 , 8 , 11 ) , phospho-antibody ( 1 , 5 , 6 , 7 , 9 , 10 ) , western blotting ( 1 , 5 , 7 , 10 , 11 )
Disease tissue studied:
bone cancer ( 5 , 8 , 10 ) , breast cancer ( 9 , 10 ) , cervical cancer ( 9 ) , lung cancer ( 1 , 4 , 5 ) , non-small cell lung cancer ( 1 , 4 , 5 ) , prostate cancer ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
CDK7 (human) ( 12 ) , Chk1 (human) ( 5 ) , Chk2 (human) ( 5 ) , PKACA (human) ( 10 ) , PKCA (human) ( 6 , 7 , 11 )
Treatments:
camptothecin ( 1 , 5 ) , ionizing_radiation ( 5 , 10 ) , UV ( 5 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 1 , 12 ) , intracellular localization ( 4 , 7 ) , molecular association, regulation ( 1 , 3 , 8 , 10 , 12 )
Effects of modification on biological processes:
cell cycle regulation ( 8 ) , chromatin organization, altered ( 7 ) , transcription, induced ( 8 )
Induce interaction with:
14-3-3 epsilon (human) ( 1 , 3 ) , 14-3-3 gamma (human) ( 1 , 3 , 8 ) , 14-3-3 zeta (human) ( 10 ) , DNA ( 3 , 10 , 12 ) , p53 (human) ( 3 )
Inhibit interaction with:
BLM (human) ( 7 ) , WRN (human) ( 7 )

References 

1

Rajagopalan S, Sade RS, Townsley FM, Fersht AR (2010) Mechanistic differences in the transcriptional activation of p53 by 14-3-3 isoforms. Nucleic Acids Res 38, 893-906
19933256   Curated Info

2

van Dieck J, et al. (2009) Posttranslational modifications affect the interaction of S100 proteins with tumor suppressor p53. J Mol Biol 394, 922-30
19819244   Curated Info

3

Rajagopalan S, et al. (2008) 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers. Nucleic Acids Res 36, 5983-91
18812399   Curated Info

4

Lee MK, Sabapathy K (2007) Phosphorylation at carboxyl-terminal S373 and S375 residues and 14-3-3 binding are not required for mouse p53 function. Neoplasia 9, 690-8
17898864   Curated Info

5

Ou YH, Chung PH, Sun TP, Shieh SY (2005) p53 C-terminal phosphorylation by CHK1 and CHK2 participates in the regulation of DNA-damage-induced C-terminal acetylation. Mol Biol Cell 16, 1684-95
15659650   Curated Info

6

Pospísilová S, et al. (2004) Activation of the DNA-binding ability of latent p53 protein by protein kinase C is abolished by protein kinase CK2. Biochem J 378, 939-47
14640983   Curated Info

7

Yang Q, et al. (2002) The processing of Holliday junctions by BLM and WRN helicases is regulated by p53. J Biol Chem 277, 31980-7
12080066   Curated Info

8

Stavridi ES, Chehab NH, Malikzay A, Halazonetis TD (2001) Substitutions that compromise the ionizing radiation-induced association of p53 with 14-3-3 proteins also compromise the ability of p53 to induce cell cycle arrest. Cancer Res 61, 7030-3
11585729   Curated Info

9

Otvos L, et al. (1998) A monoclonal antibody to a multiphosphorylated, conformational epitope at the carboxy-terminus of p53. Biochim Biophys Acta 1404, 457-74
9739174   Curated Info

10

Waterman MJ, Stavridi ES, Waterman JL, Halazonetis TD (1998) ATM-dependent activation of p53 involves dephosphorylation and association with 14-3-3 proteins. Nat Genet 19, 175-8
9620776   Curated Info

11

Youmell M, Park SJ, Basu S, Price BD (1998) Regulation of the p53 protein by protein kinase C alpha and protein kinase C zeta. Biochem Biophys Res Commun 245, 514-8
9571186   Curated Info

12

Lu H, Fisher RP, Bailey P, Levine AJ (1997) The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro. Mol Cell Biol 17, 5923-34
9315650   Curated Info

13

Sakaguchi K, et al. (1997) Phosphorylation of serine 392 stabilizes the tetramer formation of tumor suppressor protein p53. Biochemistry 36, 10117-24
9254608   Curated Info