Lys503

Site Information
LkIEVIEkLFAMDTE SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 12723510

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 3 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 3 ) , western blotting ( 3 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 3 ) , HeLa (cervical) ( 3 ) , HEp-2 (squamous) ( 1 ) , Jurkat (T lymphocyte) ( 1 )

References
1	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
2	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info
3	Paltoglou S, Roberts BJ (2007) HIF-1alpha and EPAS ubiquitination mediated by the VHL tumour suppressor involves flexibility in the ubiquitination mechanism, similar to other RING E3 ligases. Oncogene 26, 604-9 16862177 Curated Info