Ser746
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Home > Phosphorylation Site Page: > Ser746  -  EXO1 (human)

Site Information
PGLYkSSsADsLStT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 1240803

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 8 , 9 ) , mutation of modification site ( 1 , 7 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
bone cancer ( 1 ) , breast cancer ( 4 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 , 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
CAMKK2 (human) ( 1 )
Putative in vivo kinases:
AMPKA1 (mouse) ( 1 ) , Chk1 (human) ( 1 )
Kinases, in vitro:
AMPKA1 (human) ( 1 ) , Chk1 (human) ( 1 )
Treatments:
A23187 ( 1 ) , aphidicolin ( 1 ) , AZD7762 ( 1 ) , BAPTA-AM ( 1 ) , compound_C ( 1 ) , hydroxyurea ( 1 ) , ischemia ( 4 ) , siRNA ( 1 ) , thapsigargin ( 1 )

Downstream Regulation
Effects of modification on EXO1:
molecular association, regulation ( 1 , 7 ) , protein conformation ( 7 )
Effects of modification on biological processes:
chromatin organization, altered ( 1 ) , signaling pathway regulation ( 1 )
Induce interaction with:
14-3-3 beta (human) ( 7 ) , 14-3-3 eta (human) ( 7 ) , 14-3-3 gamma (human) ( 7 ) , 14-3-3 sigma (human) ( 1 , 7 )

References 

1

Li S, et al. (2019) Ca-Stimulated AMPK-Dependent Phosphorylation of Exo1 Protects Stressed Replication Forks from Aberrant Resection. Mol Cell 74, 1123-1137.e6
31053472   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

7

Andersen SD, et al. (2012) 14-3-3 checkpoint regulatory proteins interact specifically with DNA repair protein human exonuclease 1 (hEXO1) via a semi-conserved motif. DNA Repair (Amst) 11, 267-77
22222486   Curated Info

8

Moritz A (2008) CST Curation Set: 4979; Year: 2008; Biosample/Treatment: cell line, NCI-H1703/U 0126; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

9

El-Shemerly M, et al. (2008) ATR-dependent pathways control hEXO1 stability in response to stalled forks. Nucleic Acids Res 36, 511-9
18048416   Curated Info