Tyr470
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Home > Phosphorylation Site Page: > Tyr470  -  SHPS1 (human)

Site Information
AsEDtLtyADLDMVH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451114

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 , 4 , 5 , 6 ) , mutation of modification site ( 2 , 7 , 8 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Disease tissue studied:
lung cancer ( 4 ) , neuroblastoma ( 1 ) , thymic carcinoma ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) [EphB2 (human), transfection] ( 5 ) , 3T3 (fibroblast) ( 8 ) , COS (fibroblast) ( 8 ) , heart ( 6 ) , LAN-6 (neural crest) ( 1 ) , lung ( 4 ) , RAW 264.7 (macrophage) ( 7 ) , thymus ( 3 ) , VSMC ('muscle, smooth') ( 2 )

Upstream Regulation
Putative in vivo kinases:
IGF1R (human) ( 2 )
Kinases, in vitro:
IGF1R (human) ( 2 )
Treatments:
IGF-1 ( 2 ) , LPS ( 7 ) , poly(I-C) ( 7 ) , PP2 ( 7 ) , PQ401 ( 2 )

Downstream Regulation
Effects of modification on SHPS1:
molecular association, regulation ( 2 , 7 ) , phosphorylation ( 2 , 8 )
Effects of modification on biological processes:
cell growth, induced ( 2 ) , cell motility, induced ( 2 ) , signaling pathway regulation ( 2 , 7 )
Induce interaction with:
CTK (human) ( 2 ) , SHP-1 (human) ( 7 ) , SHP-2 (human) ( 2 , 7 )

References 

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Radhakrishnan Y, et al. (2011) IGF-I Stimulates Cooperative Interaction between the IGF-I Receptor and CSK Homologous Kinase that Regulates SHPS-1 Phosphorylation in Vascular Smooth Muscle Cells. Mol Endocrinol 25, 1636-49
21799000   Curated Info

3

Li Y (2011) CST Curation Set: 10459; Year: 2011; Biosample/Treatment: tissue, thymus/untreated; Disease: thymic carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Tucker M (2011) CST Curation Set: 10952; Year: 2011; Biosample/Treatment: tissue, lung/untreated; Disease: lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Jørgensen C, et al. (2009) Cell-specific information processing in segregating populations of Eph receptor ephrin-expressing cells. Science 326, 1502-9
20007894   Curated Info

6

Stokes M (2008) CST Curation Set: 4143; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Miyake A, et al. (2008) Negative regulation by SHPS-1 of Toll-like receptor-dependent proinflammatory cytokine production in macrophages. Genes Cells 13, 209-19
18233962   Curated Info

8

Stofega MR, et al. (2000) Negative regulation of growth hormone receptor/JAK2 signaling by signal regulatory protein alpha. J Biol Chem 275, 28222-9
10842184   Curated Info