Ser213
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Home > Phosphorylation Site Page: > Ser213  -  SMAD3 (human)

Site Information
NLsPNPMsPAHNNLD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451180

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 , 9 ) , immunoprecipitation ( 4 ) , mutation of modification site ( 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 ) , western blotting ( 1 , 2 , 4 , 5 , 6 , 7 , 9 )
Disease tissue studied:
breast cancer ( 1 , 3 , 7 ) , cervical cancer ( 4 ) , cervical adenocarcinoma ( 4 ) , liver cancer ( 1 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
HRas (human) ( 9 ) , MEK1 (human) ( 6 , 9 )
Putative in vivo kinases:
CDK2 (mouse) ( 8 ) , CDK4 (mouse) ( 3 , 8 )
Kinases, in vitro:
CDK8 (human) ( 4 ) , CDK9 (human) ( 4 ) , ERK2 (human) ( 4 , 9 )
Treatments:
EGF ( 5 , 9 ) , gelatin ( 6 ) , PD98059 ( 9 ) , SB203580 ( 7 ) , TGF-beta ( 5 , 6 , 7 ) , wortmannin ( 9 ) , Y27632 ( 7 )

Downstream Regulation
Effects of modification on SMAD3:
intracellular localization ( 9 )
Effects of modification on biological processes:
cell cycle regulation ( 8 ) , cell growth, altered ( 9 ) , transcription, altered ( 8 , 9 ) , transcription, inhibited ( 3 )

References 

1

Jeon WK, et al. (2015) The proinflammatory LTB4/BLT1 signal axis confers resistance to TGF-β1-induced growth inhibition by targeting Smad3 linker region. Oncotarget 6, 41650-66
26497676   Curated Info

2

Jo E, et al. (2015) Kaempferol Suppresses Transforming Growth Factor-β1-Induced Epithelial-to-Mesenchymal Transition and Migration of A549 Lung Cancer Cells by Inhibiting Akt1-Mediated Phosphorylation of Smad3 at Threonine-179. Neoplasia 17, 525-37
26297431   Curated Info

3

Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Cyclin-dependent kinase 4-mediated phosphorylation inhibits smad3 activity in cyclin d-overexpressing breast cancer cells. Mol Cancer Res 8, 1375-87
20736297   Curated Info

4

Alarcón C, et al. (2009) Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways. Cell 139, 757-69
19914168   Curated Info

5

Gao S, et al. (2009) Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Mol Cell 36, 457-68
19917253   Curated Info

6

Hayashida T, et al. (2007) MAP-kinase activity necessary for TGFbeta1-stimulated mesangial cell type I collagen expression requires adhesion-dependent phosphorylation of FAK tyrosine 397. J Cell Sci 120, 4230-40
18032789   Curated Info

7

Kamaraju AK, Roberts AB (2005) Role of Rho/ROCK and p38 MAP kinase pathways in transforming growth factor-beta-mediated Smad-dependent growth inhibition of human breast carcinoma cells in vivo. J Biol Chem 280, 1024-36
15520018   Curated Info

8

Matsuura I, et al. (2004) Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430, 226-31
15241418   Curated Info

9

Kretzschmar M, Doody J, Timokhina I, Massagué J (1999) A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Genes Dev 13, 804-16
10197981   Curated Info

10

Liu X, et al. (1997) Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Proc Natl Acad Sci U S A 94, 10669-74
9380693   Curated Info