Ser12
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Home > Phosphorylation Site Page: > Ser12  -  PKLR iso2 (rat)

Site Information
AGYLRRAsVAQLtQE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 1218250

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 1 , 3 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
E.coli (bacterial) ( 3 ) , liver ( 1 )

Upstream Regulation
Regulatory protein:
PKACA (rat) ( 1 )
Treatments:
IBMX ( 1 ) , pyruvate ( 1 ) , sildenafil ( 1 )

Downstream Regulation
Effects of modification on PKLR iso2:
enzymatic activity, inhibited ( 2 , 3 ) , molecular association, regulation ( 3 ) , protein conformation ( 3 )
Inhibit interaction with:
PKLR iso2 (rat) ( 3 )

References 

1

Gassaway BM, et al. (2019) Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production. Cell Rep 29, 3394-3404.e9
31825824   Curated Info

2

Faustova I, Loog M, Järv J (2012) Probing L-pyruvate kinase regulatory phosphorylation site by mutagenesis. Protein J 31, 592-7
22878931   Curated Info

3

Fenton AW, Tang Q (2009) An activating interaction between the unphosphorylated n-terminus of human liver pyruvate kinase and the main body of the protein is interrupted by phosphorylation. Biochemistry 48, 3816-8
19320443   Curated Info

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