Lys65

Site Information
AEKVkANkDVAsPLk SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 12294373

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:	HEp-2 (squamous) ( 1 ) , Jurkat (T lymphocyte) ( 1 , 2 ) , PC3 (prostate cell) ( 3 )

References
1	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
2	Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31 23266961 Curated Info
3	Possemato A (2009) CST Curation Set: 8587; Year: 2010; Biosample/Treatment: cell line, PC3/Velcade; Disease: prostate cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Curated Info