Ser53
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.1.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser53  -  Wee1 (human)

Site Information
GHstGEDsAFQEPDS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451131

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 ) , mutation of modification site ( 4 , 5 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 1 , 3 , 4 )
Disease tissue studied:
bone cancer ( 4 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 )
Relevant cell line - cell type - tissue:
HEK293T (epithelial) ( 5 ) , HeLa (cervical) ( 3 , 5 ) , NCI-H1299 (pulmonary) ( 1 ) , U2OS (bone cell) ( 4 )

Upstream Regulation
Regulatory protein:
CK2B (human) ( 1 )
Kinases, in vitro:
PLK1 (human) ( 3 , 5 )

Downstream Regulation
Effects of modification on Wee1:
molecular association, regulation ( 3 , 5 ) , protein degradation ( 3 , 4 , 5 )
Effects of modification on biological processes:
cell cycle regulation ( 4 )
Induce interaction with:
BTRC (human) ( 3 ) , FBW1B (human) ( 5 )

References 

1

Yde CW, et al. (2008) The regulatory beta-subunit of protein kinase CK2 regulates cell-cycle progression at the onset of mitosis. Oncogene 27, 4986-97
18469858   Curated Info

2

Rikova K (2008) CST Curation Set: 3930; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

3

Watanabe N, et al. (2005) Cyclin-dependent kinase (CDK) phosphorylation destabilizes somatic Wee1 via multiple pathways. Proc Natl Acad Sci U S A 102, 11663-8
16085715   Curated Info

4

van Vugt MA, Brás A, Medema RH (2004) Polo-like kinase-1 controls recovery from a G2 DNA damage-induced arrest in mammalian cells. Mol Cell 15, 799-811
15350223   Curated Info

5

Watanabe N, et al. (2004) M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP. Proc Natl Acad Sci U S A 101, 4419-24
15070733   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.