Tyr201
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Tyr201  -  CTLA-4 (mouse)

Site Information
SPLTTGVyVKMPPTE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450897

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 1 , 2 ) , immunoprecipitation ( 3 ) , mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 2 , 3 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:
HEK293T (epithelial) ( 2 ) , HUT-78 (T lymphocyte) ( 2 ) , Jurkat (T lymphocyte) ( 3 ) , T lymphocyte [genetic knockin] ( 1 )

Upstream Regulation
Putative in vivo kinases:
Fyn (mouse) ( 3 ) , JAK2 (human) ( 2 ) , Lck (mouse) ( 3 )
Kinases, in vitro:
JAK2 (human) ( 2 ) , Lck (mouse) ( 3 )
Treatments:
vanadate ( 3 )

Downstream Regulation
Effects of modification on CTLA-4:
intracellular localization ( 1 , 3 ) , molecular association, regulation ( 3 ) , receptor internalization, altered ( 1 )
Induce interaction with:
JAK2 (human) ( 2 ) , SHP-2 (mouse) ( 3 )

Disease / Diagnostics Relevance
Relevant diseases:
EAE ( 1 )

References 

1

Stumpf M, Zhou X, Chikuma S, Bluestone JA (2014) Tyrosine 201 of the cytoplasmic tail of CTLA-4 critically affects T regulatory cell suppressive function. Eur J Immunol 44, 1737-46
24648182   Curated Info

2

Chikuma S, Murakami M, Tanaka K, Uede T (2000) Janus kinase 2 is associated with a box 1-like motif and phosphorylates a critical tyrosine residue in the cytoplasmic region of cytotoxic T lymphocyte associated molecule-4. J Cell Biochem 78, 241-50
10842319   Curated Info

3

Chuang E, et al. (1999) Regulation of cytotoxic T lymphocyte-associated molecule-4 by Src kinases. J Immunol 162, 1270-7
9973379   Curated Info