Tyr740
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Home > Phosphorylation Site Page: > Tyr740  -  PDGFRB (human)

Site Information
TGESDGGyMDMSKDE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447520

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 15 ) , [32P] bio-synthetic labeling ( 11 , 13 ) , immunoprecipitation ( 8 , 12 , 15 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 6 , 7 , 8 , 10 , 11 , 13 , 15 ) , peptide sequencing ( 11 ) , phospho-antibody ( 8 , 13 ) , phosphopeptide mapping ( 11 , 15 ) , western blotting ( 7 , 8 , 10 , 12 , 15 )
Disease tissue studied:
breast cancer ( 8 ) , liver cancer ( 7 ) , neuroblastoma ( 1 , 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 6 ) , epithelial ( 15 ) , HepG2 (hepatic) ( 7 ) , MCF-7 (breast cell) ( 8 ) , PAE (endothelial) ( 11 , 12 , 13 ) , SK-N-BE(2) (neural crest) ( 1 , 3 , 4 , 5 ) , SMS-KCN-A (neural crest) ( 1 , 2 ) , TRMP (renal) ( 8 , 10 )

Upstream Regulation
Regulatory protein:
PIK3C2A (human) ( 7 ) , RAC1 (human) ( 7 )
Kinases, in vitro:
PDGFRB (human) ( 9 , 14 )
Phosphatases, in vitro:
PTP1B (human) ( 9 ) , SHP-2 (human) ( 9 )
Treatments:
LY294002 ( 7 ) , PDGF ( 7 , 11 , 12 , 13 , 15 ) , wortmannin ( 7 )

Downstream Regulation
Effects of modification on PDGFRB:
activity, induced ( 10 ) , enzymatic activity, induced ( 13 ) , molecular association, regulation ( 6 , 8 , 11 , 12 , 14 , 15 )
Effects of modification on biological processes:
cell cycle regulation ( 11 )
Induce interaction with:
PIK3C2A (human) ( 6 ) , PIK3CA (cow) ( 14 ) , PIK3R1 (cow) ( 14 ) , PIK3R1 (human) ( 11 , 15 ) , SHC1 (human) ( 12 ) , SHP-1 (human) ( 8 )

References 

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Guo A (2010) CST Curation Set: 10553; Year: 2010; Biosample/Treatment: cell line, SMS-KCN-A/untreated; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Guo A (2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Guo A (2010) CST Curation Set: 10549; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Guo A (2010) CST Curation Set: 10548; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Mitchell D, et al. (2007) The Lipoxin A4 receptor is coupled to SHP-2 activation: implications for regulation of receptor tyrosine kinases. J Biol Chem 282, 15606-18
17403678   Curated Info

7

Bae YS, et al. (2000) Platelet-derived growth factor-induced H(2)O(2) production requires the activation of phosphatidylinositol 3-kinase. J Biol Chem 275, 10527-31
10744745   Curated Info

8

Yu Z, et al. (1998) SHP-1 associates with both platelet-derived growth factor receptor and the p85 subunit of phosphatidylinositol 3-kinase. J Biol Chem 273, 3687-94
9452499   Curated Info

9

Klinghoffer RA, Kazlauskas A (1995) Identification of a putative Syp substrate, the PDGF beta receptor. J Biol Chem 270, 22208-17
7545675   Curated Info

10

Franke TF, et al. (1995) The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81, 727-36
7774014   Curated Info

11

Arvidsson AK, et al. (1994) Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation. Mol Cell Biol 14, 6715-26
7935391   Curated Info

12

Yokote K, et al. (1994) Direct interaction between Shc and the platelet-derived growth factor beta-receptor. J Biol Chem 269, 15337-43
8195171   Curated Info

13

Wennström S, et al. (1994) Membrane ruffling and chemotaxis transduced by the PDGF beta-receptor require the binding site for phosphatidylinositol 3' kinase. Oncogene 9, 651-60
8290276   Curated Info

14

Kazlauskas A, Kashishian A, Cooper JA, Valius M (1992) GTPase-activating protein and phosphatidylinositol 3-kinase bind to distinct regions of the platelet-derived growth factor receptor beta subunit. Mol Cell Biol 12, 2534-44
1375321   Curated Info

15

Kashishian A, Kazlauskas A, Cooper JA (1992) Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. EMBO J 11, 1373-82
1314164   Curated Info