Tyr771
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Home > Phosphorylation Site Page: > Tyr771  -  PDGFRB (human)

Site Information
ADIESSNyMAPyDNy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447517

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 10 , 18 ) , [32P] bio-synthetic labeling ( 10 , 15 ) , immunoprecipitation ( 16 , 18 ) , mass spectrometry ( 2 , 3 , 4 , 6 , 7 , 8 ) , microscopy-colocalization with upstream kinase ( 12 ) , mutation of modification site ( 5 , 9 , 11 , 14 , 15 , 18 ) , peptide sequencing ( 15 ) , phospho-antibody ( 9 , 10 , 12 ) , phosphopeptide mapping ( 10 , 15 , 18 ) , western blotting ( 11 , 12 , 14 , 16 , 18 )
Disease tissue studied:
brain cancer ( 8 ) , glioblastoma ( 8 ) , glioblastoma multiforme ( 8 ) , glioma ( 8 ) , leukemia ( 6 ) , acute myelogenous leukemia ( 6 ) , liver cancer ( 11 ) , lung cancer ( 7 ) , non-small cell lung cancer ( 7 ) , neuroblastoma ( 2 , 3 , 4 )
Relevant cell line - cell type - tissue:
epithelial ( 18 ) , F cells (fibroblast) [PDGFRB (human)] ( 12 ) , HepG2 (hepatic) ( 11 ) , lung ( 7 ) , NKM-1 (myeloid) ( 6 ) , PAE (endothelial) ( 5 , 9 , 10 , 15 , 16 ) , SK-N-BE(2) (neural crest) ( 2 , 3 , 4 ) , T98G (glial) ( 8 ) , TRMP (renal) ( 14 )

Upstream Regulation
Regulatory protein:
PIK3C2A (human) ( 11 ) , RAC1 (human) ( 11 )
Putative in vivo kinases:
PDGFRB (human) ( 10 )
Kinases, in vitro:
PDGFRB (human) ( 13 , 17 )
Putative upstream phosphatases:
PTPRJ (human) ( 10 ) , SHP-2 (human) ( 9 )
Phosphatases, in vitro:
PTP1B (human) ( 13 ) , SHP-2 (human) ( 13 )
Treatments:
LY294002 ( 11 ) , PDGF ( 5 , 9 , 10 , 11 , 12 , 16 , 18 ) , vanadate ( 9 ) , wortmannin ( 11 )

Downstream Regulation
Effects of modification on PDGFRB:
molecular association, regulation ( 9 , 15 , 16 , 17 , 18 )
Induce interaction with:
RASA1 (human) ( 9 , 15 , 17 , 18 ) , SHC1 (human) ( 16 )

References 

1

Wu K, et al. (2021) Platelet-derived growth factor receptor beta activates Abl2 via direct binding and phosphorylation. J Biol Chem 297, 100883
34144039   Curated Info

2

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

3

Guo A (2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Guo A (2010) CST Curation Set: 10549; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Wardega P, Heldin CH, Lennartsson J (2010) Mutation of tyrosine residue 857 in the PDGF beta-receptor affects cell proliferation but not migration. Cell Signal 22, 1363-8
20494825   Curated Info

6

Gu T (2006) CST Curation Set: 1414; Year: 2006; Biosample/Treatment: cell line, NKM-1/untreated; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Guo A (2006) CST Curation Set: 1249; Year: 2006; Biosample/Treatment: tissue, lung/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Stokes M (2005) CST Curation Set: 965; Year: 2005; Biosample/Treatment: cell line, T98G/serum starved; Disease: glioblastoma multiforme; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Ekman S, et al. (2002) SHP-2 is involved in heterodimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor. Oncogene 21, 1870-5
11896619   Curated Info

10

Kovalenko M, et al. (2000) Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1. J Biol Chem 275, 16219-26
10821867   Curated Info

11

Bae YS, et al. (2000) Platelet-derived growth factor-induced H(2)O(2) production requires the activation of phosphatidylinositol 3-kinase. J Biol Chem 275, 10527-31
10744745   Curated Info

12

Bernard A, Kazlauskas A (1999) Phosphospecific antibodies reveal temporal regulation of platelet-derived growth factor beta receptor signaling. Exp Cell Res 253, 704-12
10585294   Curated Info

13

Klinghoffer RA, Kazlauskas A (1995) Identification of a putative Syp substrate, the PDGF beta receptor. J Biol Chem 270, 22208-17
7545675   Curated Info

14

Franke TF, et al. (1995) The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81, 727-36
7774014   Curated Info

15

Arvidsson AK, et al. (1994) Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation. Mol Cell Biol 14, 6715-26
7935391   Curated Info

16

Yokote K, et al. (1994) Direct interaction between Shc and the platelet-derived growth factor beta-receptor. J Biol Chem 269, 15337-43
8195171   Curated Info

17

Kazlauskas A, Kashishian A, Cooper JA, Valius M (1992) GTPase-activating protein and phosphatidylinositol 3-kinase bind to distinct regions of the platelet-derived growth factor receptor beta subunit. Mol Cell Biol 12, 2534-44
1375321   Curated Info

18

Kashishian A, Kazlauskas A, Cooper JA (1992) Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. EMBO J 11, 1373-82
1314164   Curated Info