Thr55
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Home > Phosphorylation Site Page: > Thr55  -  p53 (human)

Site Information
DDIEQWFtEDPGPDE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450685

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , immunoprecipitation ( 5 , 9 ) , mutation of modification site ( 5 , 9 , 11 , 13 , 14 , 16 , 17 , 19 , 20 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 11 , 13 , 14 , 16 , 17 , 18 , 19 , 20 ) , phosphoamino acid analysis ( 13 ) , western blotting ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 11 , 13 , 14 , 16 , 17 , 18 , 20 )
Disease tissue studied:
bone cancer ( 5 , 6 , 8 , 9 , 11 , 13 , 14 , 17 , 20 ) , breast cancer ( 16 , 18 ) , colorectal cancer ( 3 , 6 , 7 , 14 , 17 ) , colorectal carcinoma ( 3 , 6 , 7 , 14 , 17 ) , leukemia ( 17 ) , T cell leukemia ( 17 ) , liver cancer ( 1 ) , lung cancer ( 11 , 13 , 14 ) , non-small cell lung cancer ( 11 , 13 , 14 ) , lymphoma ( 7 , 17 ) , anaplastic large cell lymphoma ( 17 ) , Burkitt's lymphoma ( 7 ) , neuroblastoma ( 11 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 9 ) , CCRF-CEM (T lymphocyte) ( 17 ) , DLD1 (intestinal) ( 3 ) , HAEC (endothelial) ( 2 ) , HCT116 (intestinal) ( 3 , 6 , 14 , 17 ) , heart [GRK5 (mouse), homozygous knockout] ( 9 ) , hepatocyte-liver ( 1 ) , HepG2 (hepatic) ( 1 ) , HUVEC (endothelial) ( 2 ) , IMR32 (neural crest) ( 11 ) , liver [GRK5 (mouse), homozygous knockout] ( 9 ) , lung [GRK5 (mouse), homozygous knockout] ( 9 ) , MCF-7 (breast cell) ( 16 , 18 ) , MDST8 (intestinal) ( 7 ) , Namalwa (B lymphocyte) ( 7 ) , NCI-H1299 (pulmonary) ( 11 , 13 , 14 ) , Saos-2 (bone cell) ( 9 , 17 , 20 ) , SiHa (squamous) ( 19 ) , spleen [GRK5 (mouse), homozygous knockout] ( 9 ) , SW480 (intestinal) ( 3 ) , U2OS (bone cell) [GR (human)] ( 17 ) , U2OS (bone cell) ( 5 , 6 , 8 , 11 , 13 , 14 )

Upstream Regulation
Regulatory protein:
ATM (human) ( 13 ) , PPP2CA (human) ( 7 ) , PPP2R5A (human) ( 14 ) , PPP2R5C (human) ( 8 ) , small T antigen (polyomavirus) ( 14 ) , TAF1 (human) ( 11 )
Putative in vivo kinases:
ERK2 (human) ( 19 ) , GRK5 (human) ( 9 ) , TAF1 (human) ( 14 , 17 )
Kinases, in vitro:
ERK2 (human) ( 19 ) , GRK5 (human) ( 4 , 9 ) , TAF1 (human) ( 2 , 5 , 17 )
Putative upstream phosphatases:
PPP2CA (human) ( 6 , 8 ) , PPP2R5C (human) ( 1 , 14 )
Phosphatases, in vitro:
PPP2R5C (human) ( 14 )
Treatments:
4-AN ( 5 ) , adriamycin ( 16 , 19 ) , apigenin ( 2 , 11 , 17 ) , caffeine ( 13 ) , calyculin_A ( 14 ) , compound_C ( 5 ) , high_glucose ( 2 ) , ionizing_radiation ( 13 , 14 , 17 , 18 ) , KU-55933 ( 13 ) , lenalidomide ( 7 ) , leptomycin_B ( 11 ) , MG132 ( 3 , 13 ) , NSC59984 ( 3 ) , nutlin-3 ( 3 ) , okadaic_acid ( 14 ) , PD98059 ( 16 , 19 ) , siRNA ( 1 , 11 , 14 ) , U0126 ( 16 ) , UV ( 5 , 13 , 14 , 17 ) , virus infection ( 1 ) , wortmannin ( 13 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 16 , 19 ) , activity, inhibited ( 1 ) , intracellular localization ( 11 ) , molecular association, regulation ( 2 , 4 , 5 , 11 , 15 , 17 ) , protein degradation ( 7 , 14 , 17 )
Effects of modification on biological processes:
apoptosis, altered ( 9 , 11 , 14 , 16 ) , apoptosis, inhibited ( 1 ) , carcinogenesis, induced ( 6 ) , cell cycle regulation ( 1 , 14 , 17 ) , cell growth, induced ( 6 , 14 ) , signaling pathway regulation ( 1 ) , transcription, altered ( 17 , 19 ) , transcription, induced ( 12 ) , transcription, inhibited ( 2 , 5 , 6 )
Induce interaction with:
Exportin-1 (human) ( 11 ) , GTF2H1 (human) ( 4 , 12 , 15 ) , MDM2 (human) ( 17 ) , TFB1M (human) ( 15 )
Inhibit interaction with:
DNA ( 2 , 5 )

References 

1

He C, et al. (2018) ER stress regulating protein phosphatase 2A-B56γ, targeted by hepatitis B virus X protein, induces cell cycle arrest and apoptosis of hepatocytes. Cell Death Dis 9, 762
29988038   Curated Info

2

Wu Y, et al. (2017) High glucose-induced p53 phosphorylation contributes to impairment of endothelial antioxidant system. Biochim Biophys Acta 1863, 2355-2362
28673515   Curated Info

3

Zhang S, et al. (2015) Small-Molecule NSC59984 Restores p53 Pathway Signaling and Antitumor Effects against Colorectal Cancer via p73 Activation and Degradation of Mutant p53. Cancer Res 75, 3842-52
26294215   Curated Info

4

Okuda M, Nishimura Y (2015) Real-time and simultaneous monitoring of the phosphorylation and enhanced interaction of p53 and XPC acidic domains with the TFIIH p62 subunit. Oncogenesis 4, e150
26029824   Curated Info

5

Wu Y, et al. (2014) Phosphorylation of p53 by TAF1 inactivates p53-dependent transcription in the DNA damage response. Mol Cell 53, 63-74
24289924   Curated Info

6

Nobumori Y, et al. (2013) B56γ tumor-associated mutations provide new mechanisms for B56γ-PP2A tumor suppressor activity. Mol Cancer Res 11, 995-1003
23723076   Curated Info

7

Wei S, et al. (2013) Lenalidomide promotes p53 degradation by inhibiting MDM2 auto-ubiquitination in myelodysplastic syndrome with chromosome 5q deletion. Oncogene 32, 1110-20
22525275   Curated Info

8

Shouse GP, Nobumori Y, Liu X (2010) A B56gamma mutation in lung cancer disrupts the p53-dependent tumor-suppressor function of protein phosphatase 2A. Oncogene 29, 3933-41
20473327   Curated Info

9

Chen X, et al. (2010) G-protein-coupled receptor kinase 5 phosphorylates p53 and inhibits DNA damage-induced apoptosis. J Biol Chem 285, 12823-30
20124405   Curated Info

10

van Dieck J, et al. (2009) Posttranslational modifications affect the interaction of S100 proteins with tumor suppressor p53. J Mol Biol 394, 922-30
19819244   Curated Info

11

Cai X, Liu X (2008) Inhibition of Thr-55 phosphorylation restores p53 nuclear localization and sensitizes cancer cells to DNA damage. Proc Natl Acad Sci U S A 105, 16958-63
18952844   Curated Info

12

Di Lello P, et al. (2008) p53 and TFIIEalpha share a common binding site on the Tfb1/p62 subunit of TFIIH. Proc Natl Acad Sci U S A 105, 106-11
18160537   Curated Info

13

Shouse GP, Cai X, Liu X (2008) Serine 15 phosphorylation of p53 directs its interaction with B56gamma and the tumor suppressor activity of B56gamma-specific protein phosphatase 2A. Mol Cell Biol 28, 448-56
17967874   Curated Info

14

Li HH, et al. (2007) A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55. EMBO J 26, 402-11
17245430   Curated Info

15

Di Lello P, et al. (2006) Structure of the Tfb1/p53 complex: Insights into the interaction between the p62/Tfb1 subunit of TFIIH and the activation domain of p53. Mol Cell 22, 731-40
16793543   Curated Info

16

Yeh PY, et al. (2004) Phosphorylation of p53 on Thr55 by ERK2 is necessary for doxorubicin-induced p53 activation and cell death. Oncogene 23, 3580-8
15116093   Curated Info

17

Li HH, Li AG, Sheppard HM, Liu X (2004) Phosphorylation on Thr-55 by TAF1 mediates degradation of p53: a role for TAF1 in cell G1 progression. Mol Cell 13, 867-78
15053879   Curated Info

18

Xia L, Paik A, Li JJ (2004) p53 activation in chronic radiation-treated breast cancer cells: regulation of MDM2/p14ARF. Cancer Res 64, 221-8
14729628   Curated Info

19

Yeh PY, et al. (2001) Nuclear extracellular signal-regulated kinase 2 phosphorylates p53 at Thr55 in response to doxorubicin. Biochem Biophys Res Commun 284, 880-6
11409876   Curated Info

20

Gatti A, Li HH, Traugh JA, Liu X (2000) Phosphorylation of human p53 on Thr-55. Biochemistry 39, 9837-42
10933801   Curated Info