Tyr228
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Home > Phosphorylation Site Page: > Tyr228  -  RACK1 (human)

Site Information
LNEGkHLyTLDGGDI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450543
Available spectra:  1 CST
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 2 , 3 , 8 ) , phospho-antibody ( 8 ) , western blotting ( 8 )
Disease tissue studied:
colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , leukemia ( 4 ) , acute myelogenous leukemia ( 4 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 ) , lymphoma ( 6 , 7 ) , anaplastic large cell lymphoma ( 6 , 7 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Src (human) ( 8 )
Kinases, in vitro:
Src (human) ( 8 )

Downstream Regulation
Effects of modification on RACK1:
intracellular localization ( 3 ) , molecular association, regulation ( 8 ) , protein stabilization ( 3 ) , ubiquitination ( 3 )
Effects of modification on biological processes:
carcinogenesis, inhibited ( 3 ) , cell adhesion, induced ( 3 ) , cell motility, inhibited ( 3 ) , endocytosis, inhibited ( 3 )

References 

1

Rikova K (2013) CST Curation Set: 18449; Year: 2012; Biosample/Treatment: cell line, H2228/crizotinib, geldanamycin; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine (P-Tyr-1000) MultiMab(TM) Rabbit mAb mix Cat#: 8954
Curated Info

2

Yang J, et al. (2012) Receptor for Activated C Kinase 1 (RACK1) Inhibits Function of Transient Receptor Potential (TRP)-type Channel Pkd2L1 through Physical Interaction. J Biol Chem 287, 6551-61
22174419   Curated Info

3

Swaminathan G, Cartwright CA (2012) Rack1 promotes epithelial cell-cell adhesion by regulating E-cadherin endocytosis. Oncogene 31, 376-89
21685945   Curated Info

4

Goss V (2005) CST Curation Set: 964; Year: 2005; Biosample1/Treatment/Isotope: cell line, CTV-1/PP2/L, Biosample2/Treatment/Isotope: cell line, CTV-1/none/H; Disease: acute myelogenous leukemia; SILAC: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Rush J, et al. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nat Biotechnol 23, 94-101
15592455   Curated Info

6

Moritz A (2004) CST Curation Set: 398; Year: 2004; Biosample/Treatment: cell line, SU-DHL1/-; Disease: anaplastic large cell lymphoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Moritz A (2003) CST Curation Set: 182; Year: 2003; Biosample/Treatment: cell line, SU-DHL1/-; Disease: anaplastic large cell lymphoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Chang BY, Harte RA, Cartwright CA (2002) RACK1: a novel substrate for the Src protein-tyrosine kinase. Oncogene 21, 7619-29
12400005   Curated Info