Thr505
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Home > Phosphorylation Site Page: > Thr505  -  PKCD (mouse)

Site Information
FGEGRAstFCGtPDY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447511

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 18 , 20 , 21 , 22 , 23 ) , mutation of modification site ( 17 , 25 ) , phospho-antibody ( 3 , 17 , 19 , 24 , 25 , 26 , 27 ) , phosphoamino acid analysis ( 28 ) , western blotting ( 3 , 17 , 19 , 25 , 28 )
Disease tissue studied:
anthrax infection ( 5 ) , bladder cancer ( 28 ) , leukemia ( 14 , 15 , 16 ) , fibrosarcoma of soft tissue ( 25 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 26 , 28 ) , 3T3-L1 (fibroblast) ( 13 , 18 ) , BHK (fibroblast) ( 24 ) , brain ( 2 , 6 , 7 ) , COS (fibroblast) ( 17 ) , embryo ( 2 ) , HEK293T (epithelial) ( 3 ) , kidney ( 6 ) , liver ( 1 , 2 , 9 , 10 , 11 , 12 ) , liver [leptin (mouse), homozygous knockout] ( 1 ) , lung ( 6 ) , mast-bone marrow ( 27 ) , MEF (fibroblast) ( 3 , 19 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 4 ) , mpkCCD (renal) ( 8 ) , myocyte-heart ( 19 ) , pancreas ( 6 ) , platelet-blood ( 17 ) , RAW 264.7 (macrophage) ( 14 , 15 , 16 , 25 ) , spleen ( 5 , 6 ) , SYF (fibroblast) ( 19 ) , testis ( 6 ) , U3A (fibroblast) ( 25 ) , UMUC3 (bladder cell) ( 28 )

Upstream Regulation
Treatments:
antigenic stimulation ( 27 ) , cell_adhesion ( 28 ) , collagen_IV ( 26 ) , DiC8 ( 17 ) , dioctanoylglycerol ( 24 ) , endothelin ( 19 ) , fibronectin ( 26 ) , H2O2 ( 19 ) , IFN-gamma ( 25 ) , linoleic_acid ( 24 ) , LY294002 ( 28 ) , norepinephrine ( 19 ) , OAG ( 24 ) , PAR4-activating_peptide ( 17 ) , PDGF ( 19 ) , phorbol_ester ( 19 ) , PP2 ( 17 ) , serum ( 28 ) , stearic_acid ( 24 ) , thrombin ( 3 ) , U73122 ( 19 )

References 

1

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

2

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

3

Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55
21868363   Curated Info

4

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

5

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

6

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

7

Possemato A (2010) CST Curation Set: 9877; Year: 2010; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

8

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

9

Possemato A (2009) CST Curation Set: 8040; Year: 2011; Biosample/Treatment: tissue, liver/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

10

Possemato A (2009) CST Curation Set: 8043; Year: 2011; Biosample/Treatment: tissue, liver/AICAR; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

11

Possemato A (2009) CST Curation Set: 8042; Year: 2011; Biosample/Treatment: tissue, liver/AICAR; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

12

Possemato A (2009) CST Curation Set: 8041; Year: 2011; Biosample/Treatment: tissue, liver/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

13

Guo A (2009) CST Curation Set: 7449; Year: 2009; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

14

Possemato A (2009) CST Curation Set: 6932; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/MG132; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

15

Possemato A (2009) CST Curation Set: 6930; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/LPS; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

16

Possemato A (2009) CST Curation Set: 6929; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/control; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

17

Murugappan S, et al. (2009) Differential regulation of threonine and tyrosine phosphorylations on protein kinase Cdelta by G-protein-mediated pathways in platelets. Biochem J 417, 113-20
18652571   Curated Info

18

Possemato A (2008) CST Curation Set: 4744; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

19

Rybin VO, et al. (2008) Phorbol 12-myristate 13-acetate-dependent protein kinase C delta-Tyr311 phosphorylation in cardiomyocyte caveolae. J Biol Chem 283, 17777-88
18387943   Curated Info

20

Rikova K (2008) CST Curation Set: 4511; Year: 2008; Biosample/Treatment: cell line, MD Ander 1249/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

21

Rikova K (2008) CST Curation Set: 4512; Year: 2008; Biosample/Treatment: cell line, MD Ander a0859/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

22

Rikova K (2008) CST Curation Set: 4506; Year: 2008; Biosample/Treatment: cell line, MD Ander 1169/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

23

Rikova K (2008) CST Curation Set: 4507; Year: 2008; Biosample/Treatment: cell line, MD Ander 1158(393P)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

24

Wang Y, Oram JF (2007) Unsaturated fatty acids phosphorylate and destabilize ABCA1 through a protein kinase C delta pathway. J Lipid Res 48, 1062-8
17325386   Curated Info

25

Kwon MJ, et al. (2007) Role of PKCdelta in IFN-gamma-inducible CIITA gene expression. Mol Immunol 44, 2841-9
17346795   Curated Info

26

Ivaska J, Bosca L, Parker PJ (2003) PKCepsilon is a permissive link in integrin-dependent IFN-gamma signalling that facilitates JAK phosphorylation of STAT1. Nat Cell Biol 5, 363-9
12640464   Curated Info

27

Leitges M, et al. (2002) Protein kinase C-delta is a negative regulator of antigen-induced mast cell degranulation. Mol Cell Biol 22, 3970-80
12024011   Curated Info

28

Parekh DB, et al. (2000) Beta1-integrin and PTEN control the phosphorylation of protein kinase C. Biochem J 352 Pt 2, 425-33
11085936   Curated Info