Thr505
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Home > Phosphorylation Site Page: > Thr505  -  PKCD (rat)

Site Information
FGENRAstFCGTPDy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447511

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 12 ) , immunoprecipitation ( 14 ) , mass spectrometry ( 1 , 2 , 3 , 13 ) , mutation of modification site ( 6 , 13 ) , phospho-antibody ( 1 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ) , western blotting ( 4 , 6 , 7 , 8 , 10 , 12 , 13 , 14 )
Disease tissue studied:
adrenal cancer ( 3 ) , pheochromocytoma ( 3 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 11 ) , 293 (epithelial) [PDK1 (human)] ( 15 ) , 293 (epithelial) [PKCD (rat)] ( 15 ) , 293 (epithelial) ( 10 ) , cardiac ( 8 ) , COS (fibroblast) ( 13 ) , fibroblast-heart ( 7 ) , heart ( 4 ) , IEC-6 (epithelial) ( 2 ) , MEF (fibroblast) [PKCE (mouse)] ( 8 ) , myocyte-heart ( 1 , 6 , 7 , 9 , 12 ) , parotid acinar-salivary gland ( 14 ) , PC-12 (chromaffin) ( 3 ) , SYF (fibroblast) ( 8 )

Upstream Regulation
Regulatory protein:
PKCD (rat) ( 8 ) , PKCE (mouse) ( 8 )
Putative in vivo kinases:
PDK1 (human) ( 15 ) , PKCE (mouse) ( 8 )
Kinases, in vitro:
PDK1 (human) ( 15 )
Phosphatases, in vitro:
PTP1B (human) ( 14 )
Treatments:
carbachol ( 14 ) , EGF ( 5 ) , endothelin ( 9 ) , GF109203X ( 7 , 8 , 9 , 10 ) , Go_6976 ( 7 , 8 ) , H-CHO ( 11 ) , H2O2 ( 7 , 8 , 10 , 13 ) , insulin ( 11 ) , isoproterenol ( 7 ) , leucine ( 11 ) , LY294002 ( 8 , 9 , 14 ) , meal feeding ( 11 ) , norepinephrine ( 7 , 8 , 12 ) , norleucine ( 11 ) , Pasteurella_multocida_toxin ( 7 ) , PDGF ( 9 ) , phorbol_ester ( 7 , 8 , 9 , 10 , 12 , 14 ) , PP2 ( 9 ) , prazosin ( 7 ) , propranolol ( 7 ) , rapamycin ( 14 ) , streptozotocin ( 4 ) , thrombin ( 7 ) , U0126 ( 7 ) , UCN-01 ( 8 ) , wortmannin ( 14 )

Downstream Regulation
Effects of modification on PKCD:
enzymatic activity, induced ( 6 , 9 )

References 

1

Gong J, Holewinski RJ, Van Eyk JE, Steinberg SF (2016) A novel phosphorylation site at Ser130 adjacent to the pseudosubstrate domain contributes to the activation of protein kinase C-δ. Biochem J 473, 311-20
26546672   Curated Info

2

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

3

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

4

Lin G, Liu Y, MacLeod KM (2009) Regulation of muscle creatine kinase by phosphorylation in normal and diabetic hearts. Cell Mol Life Sci 66, 135-44
18979206   Curated Info

5

Philippar U, et al. (2008) A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis. Dev Cell 15, 813-28
19081071   Curated Info

6

Sumandea MP, et al. (2008) Tyrosine phosphorylation modifies protein kinase C delta-dependent phosphorylation of cardiac troponin I. J Biol Chem 283, 22680-9
18550549   Curated Info

7

Ozgen N, et al. (2008) Protein kinase D links Gq-coupled receptors to cAMP response element-binding protein (CREB)-Ser133 phosphorylation in the heart. J Biol Chem 283, 17009-19
18378685   Curated Info

8

Rybin VO, et al. (2007) Protein kinase Cepsilon (PKCepsilon) and Src control PKCdelta activation loop phosphorylation in cardiomyocytes. J Biol Chem 282, 23631-8
17569658   Curated Info

9

Markou T, Yong CS, Sugden PH, Clerk A (2006) Regulation of protein kinase C delta by phorbol ester, endothelin-1, and platelet-derived growth factor in cardiac myocytes. J Biol Chem 281, 8321-31
16361709   Curated Info

10

Rybin VO, Steinberg SF (2006) Immunoblotting PKC-delta: a cautionary note from the bench. Am J Physiol Cell Physiol 290, C750-6
16236821   Curated Info

11

Vary TC, Goodman S, Kilpatrick LE, Lynch CJ (2005) Nutrient regulation of PKCepsilon is mediated by leucine, not insulin, in skeletal muscle. Am J Physiol Endocrinol Metab 289, E684-94
15886222   Curated Info

12

Rybin VO, et al. (2004) Stimulus-specific differences in protein kinase C delta localization and activation mechanisms in cardiomyocytes. J Biol Chem 279, 19350-61
14970215   Curated Info

13

Konishi H, et al. (2001) Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro. Proc Natl Acad Sci U S A 98, 6587-92
11381116   Curated Info

14

Benes C, Soltoff SP (2001) Modulation of PKCdelta tyrosine phosphorylation and activity in salivary and PC-12 cells by Src kinases. Am J Physiol Cell Physiol 280, C1498-510
11350745   Curated Info

15

Le Good JA, et al. (1998) Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1. Science 281, 2042-5
9748166   Curated Info