Ser59
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Home > Phosphorylation Site Page: > Ser59  -  14-3-3 eta (mouse)

Site Information
VVGARRSsWRVIssI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450482

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 ) , phospho-antibody ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
PKACA (cow) ( 4 ) , PKCD (rat) ( 5 )
Treatments:
cAMP_analog ( 4 ) , DMS ( 4 ) , GF109203X ( 4 ) , H-89 ( 4 ) , mPKI ( 4 )

References 

1

Possemato A (2008) CST Curation Set: 3852; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

2

Possemato A (2008) CST Curation Set: 3854; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

3

Possemato A (2008) CST Curation Set: 3841; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

4

Ma Y, et al. (2005) Sphingosine activates protein kinase A type II by a novel cAMP-independent mechanism. J Biol Chem 280, 26011-7
15883165   Curated Info

5

Hamaguchi A, et al. (2003) A sphingosine-dependent protein kinase that specifically phosphorylates 14-3-3 (SDK1) is identified as the kinase domain of PKCdelta: a preliminary note. Biochem Biophys Res Commun 307, 589-94
12893264   Curated Info