Tyr751
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Home > Phosphorylation Site Page: > Tyr751  -  PDGFRB (human)

Site Information
SKDESVDyVPMLDMK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447508

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 13 , 24 ) , [32P] bio-synthetic labeling ( 13 , 19 , 21 , 25 ) , immunoprecipitation ( 2 , 16 , 20 , 24 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 ) , microscopy-colocalization with upstream kinase ( 15 ) , mutation of modification site ( 9 , 10 , 12 , 14 , 16 , 18 , 19 , 21 , 24 , 25 ) , peptide sequencing ( 19 ) , phospho-antibody ( 2 , 8 , 11 , 12 , 13 , 15 , 16 , 21 ) , phosphopeptide mapping ( 13 , 19 , 24 , 25 ) , western blotting ( 2 , 8 , 11 , 12 , 14 , 15 , 16 , 18 , 20 , 24 )
Disease tissue studied:
breast cancer ( 8 , 16 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , liver cancer ( 12 , 14 ) , neuroblastoma ( 3 , 4 , 5 , 6 , 7 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 10 ) , 3T3 (fibroblast) ( 12 ) , AG1523 (fibroblast) [PDGFRB (human)] ( 25 ) , breast ( 1 ) , epithelial ( 24 ) , F cells (fibroblast) [PDGFRB (human)] ( 15 ) , glial-brain ( 2 ) , HepG2 (hepatic) ( 12 , 14 ) , MCF-7 (breast cell) ( 16 ) , MDA-MB-231 (breast cell) ( 8 ) , PAE (endothelial) ( 9 , 13 , 19 , 20 , 21 ) , SK-N-BE(2) (neural crest) ( 3 , 5 , 6 , 7 ) , SMS-KCN-A (neural crest) ( 3 , 4 ) , TRMP (renal) ( 16 , 18 ) , vascular smooth muscle cell ('muscle, smooth') ( 11 )

Upstream Regulation
Regulatory protein:
PDGFRB (human) ( 25 ) , PIK3C2A (human) ( 14 ) , RAC1 (human) ( 14 )
Putative in vivo kinases:
PDGFRB (human) ( 13 , 25 )
Kinases, in vitro:
PDGFRB (human) ( 17 , 23 , 25 )
Putative upstream phosphatases:
ACP1 (human) ( 12 ) , PTPRJ (human) ( 13 )
Phosphatases, in vitro:
PTP1B (human) ( 17 ) , PTPRJ (human) ( 13 ) , SHP-2 (human) ( 17 )
Treatments:
4-HT ( 8 ) , 9cRA ( 8 ) , angiotensin ( 11 ) , dexamethasone ( 8 ) , LY294002 ( 14 ) , PDGF ( 12 , 13 , 14 , 15 , 19 , 20 , 21 , 24 , 25 ) , retinoic_acid ( 8 ) , tamoxifen ( 8 ) , wortmannin ( 14 )

Downstream Regulation
Effects of modification on PDGFRB:
activity, induced ( 18 ) , enzymatic activity, induced ( 21 ) , molecular association, regulation ( 10 , 16 , 19 , 20 , 22 , 23 , 24 ) , protein conformation ( 22 )
Effects of modification on biological processes:
cell cycle regulation ( 19 ) , signaling pathway regulation ( 2 )
Induce interaction with:
PIK3C2A (human) ( 10 ) , PIK3CA (cow) ( 23 ) , PIK3R1 (cow) ( 23 ) , PIK3R1 (human) ( 19 , 22 , 24 ) , SHC1 (human) ( 20 ) , SHP-1 (human) ( 16 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Zhang J, et al. (2015) PDGFR-β-activated ACK1-AKT Signaling Promotes Glioma Tumorigenesis. Int J Cancer 136, 1769-80
25257795   Curated Info

3

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

4

Guo A (2010) CST Curation Set: 10553; Year: 2010; Biosample/Treatment: cell line, SMS-KCN-A/untreated; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Guo A (2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Guo A (2010) CST Curation Set: 10549; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Guo A (2010) CST Curation Set: 10548; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Eto I (2010) Upstream molecular signaling pathways of p27(Kip1) expression: Effects of 4-hydroxytamoxifen, dexamethasone, and retinoic acids. Cancer Cell Int 10, 3
20170512   Curated Info

9

Ruusala A, Pawson T, Heldin CH, Aspenström P (2008) Nck adapters are involved in the formation of dorsal ruffles, cell migration, and Rho signaling downstream of the platelet-derived growth factor beta receptor. J Biol Chem 283, 30034-44
18765673   Curated Info

10

Mitchell D, et al. (2007) The Lipoxin A4 receptor is coupled to SHP-2 activation: implications for regulation of receptor tyrosine kinases. J Biol Chem 282, 15606-18
17403678   Curated Info

11

Gao BB, Hansen H, Chen HC, Feener EP (2006) Angiotensin II stimulates phosphorylation of an ectodomain-truncated platelet-derived growth factor receptor-beta and its binding to class IA PI3K in vascular smooth muscle cells. Biochem J 397, 337-44
16569213   Curated Info

12

Chiarugi P, et al. (2002) Insight into the role of low molecular weight phosphotyrosine phosphatase (LMW-PTP) on platelet-derived growth factor receptor (PDGF-r) signaling. LMW-PTP controls PDGF-r kinase activity through TYR-857 dephosphorylation. J Biol Chem 277, 37331-8
12149261   Curated Info

13

Kovalenko M, et al. (2000) Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1. J Biol Chem 275, 16219-26
10821867   Curated Info

14

Bae YS, et al. (2000) Platelet-derived growth factor-induced H(2)O(2) production requires the activation of phosphatidylinositol 3-kinase. J Biol Chem 275, 10527-31
10744745   Curated Info

15

Bernard A, Kazlauskas A (1999) Phosphospecific antibodies reveal temporal regulation of platelet-derived growth factor beta receptor signaling. Exp Cell Res 253, 704-12
10585294   Curated Info

16

Yu Z, et al. (1998) SHP-1 associates with both platelet-derived growth factor receptor and the p85 subunit of phosphatidylinositol 3-kinase. J Biol Chem 273, 3687-94
9452499   Curated Info

17

Klinghoffer RA, Kazlauskas A (1995) Identification of a putative Syp substrate, the PDGF beta receptor. J Biol Chem 270, 22208-17
7545675   Curated Info

18

Franke TF, et al. (1995) The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81, 727-36
7774014   Curated Info

19

Arvidsson AK, et al. (1994) Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation. Mol Cell Biol 14, 6715-26
7935391   Curated Info

20

Yokote K, et al. (1994) Direct interaction between Shc and the platelet-derived growth factor beta-receptor. J Biol Chem 269, 15337-43
8195171   Curated Info

21

Wennström S, et al. (1994) Membrane ruffling and chemotaxis transduced by the PDGF beta-receptor require the binding site for phosphatidylinositol 3' kinase. Oncogene 9, 651-60
8290276   Curated Info

22

Panayotou G, et al. (1992) Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes. EMBO J 11, 4261-72
1330535   Curated Info

23

Kazlauskas A, Kashishian A, Cooper JA, Valius M (1992) GTPase-activating protein and phosphatidylinositol 3-kinase bind to distinct regions of the platelet-derived growth factor receptor beta subunit. Mol Cell Biol 12, 2534-44
1375321   Curated Info

24

Kashishian A, Kazlauskas A, Cooper JA (1992) Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. EMBO J 11, 1373-82
1314164   Curated Info

25

Kazlauskas A, Cooper JA (1989) Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins. Cell 58, 1121-33
2550144   Curated Info