Ser491
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Home > Phosphorylation Site Page: > Ser491  -  AMPKA2 (rat)

Site Information
STPQRSCsAAGLHRP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450432

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 4 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:
Chang liver (cervical) ( 4 ) , heart ( 3 ) , liver ( 1 ) , myocyte-heart ( 2 )

Upstream Regulation
Regulatory protein:
Akt1 (rat) ( 2 ) , LKB1 (human) ( 2 )
Putative in vivo kinases:
Akt1 (rat) ( 3 )
Kinases, in vitro:
LKB1 (mouse) ( 4 )
Treatments:
hypoxia ( 2 ) , insulin ( 3 ) , ischemia ( 3 ) , phenobarbital ( 1 )

Downstream Regulation
Effects of modification on AMPKA2:
enzymatic activity, inhibited ( 1 )

References 

1

Shindo S, Numazawa S, Yoshida T (2007) A physiological role of AMP-activated protein kinase in phenobarbital-mediated constitutive androstane receptor activation and CYP2B induction. Biochem J 401, 735-41
17032173   Curated Info

2

Soltys CL, Kovacic S, Dyck JR (2006) Activation of cardiac AMP-activated protein kinase by LKB1 expression or chemical hypoxia is blunted by increased Akt activity. Am J Physiol Heart Circ Physiol 290, H2472-9
16428351   Curated Info

3

Horman S, et al. (2006) Insulin antagonizes ischemia-induced Thr172 phosphorylation of AMP-activated protein kinase alpha-subunits in heart via hierarchical phosphorylation of Ser485/491. J Biol Chem 281, 5335-40
16340011   Curated Info

4

Woods A, et al. (2003) Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis. J Biol Chem 278, 28434-42
12764152   Curated Info