Ser215
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.0.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser215  -  NRF2 (human)

Site Information
VETTMVPsPEAKLTE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 10292704

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 8 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , mutation of modification site ( 1 , 8 ) , phospho-antibody ( 8 ) , western blotting ( 8 )
Disease tissue studied:
breast cancer ( 1 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 ) , cervical cancer ( 7 ) , cervical adenocarcinoma ( 7 ) , melanoma skin cancer ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 ) , breast ( 3 ) , HEK293T (epithelial) ( 1 , 8 ) , HeLa (cervical) ( 5 , 6 ) , HeLa S3 (cervical) ( 7 ) , K562 (erythroid) ( 6 ) , MCF10A1 (epithelial) ( 1 ) , MDA-MB-231 (breast cell) ( 1 ) , MEF (fibroblast) ( 1 , 2 ) , WM239A (melanocyte) ( 4 )

Upstream Regulation
Putative in vivo kinases:
ERK1 ( 1 ) , JNK1 (human) ( 1 )
Treatments:
SP600125 ( 1 ) , U0126 ( 1 )

Downstream Regulation
Effects of modification on NRF2:
intracellular localization ( 8 ) , molecular association, regulation ( 1 ) , protein stabilization ( 1 )
Effects of modification on biological processes:
transcription, induced ( 8 )
Induce interaction with:
PIN1 (human) ( 1 )

References 

1

Saeidi S, et al. (2022) Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 directly binds and stabilizes Nrf2 in breast cancer. FASEB J 36, e22068
34918396   Curated Info

2

Matzinger M, et al. (2020) AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Redox Biol 29, 101393
31805502   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

8

Sun Z, Huang Z, Zhang DD (2009) Phosphorylation of Nrf2 at multiple sites by MAP kinases has a limited contribution in modulating the Nrf2-dependent antioxidant response. PLoS One 4, e6588
19668370   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.