Ser408

Site Information
GDMVQPLsPSQGQST SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 10292700

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 , 5 ) , mass spectrometry ( 2 , 3 , 4 , 5 ) , mutation of modification site ( 1 , 5 ) , phospho-antibody ( 5 ) , western blotting ( 2 , 5 )
Disease tissue studied:	breast cancer ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 2 ) , HEK293T (epithelial) ( 1 , 5 ) , HeLa (cervical) ( 3 ) , Jurkat (T lymphocyte) ( 4 ) , MCF10A1 (epithelial) ( 1 ) , MDA-MB-231 (breast cell) ( 1 ) , MEF (fibroblast) ( 1 , 2 )

Downstream Regulation
Effects of modification on NRF2:	activity, induced ( 2 ) , intracellular localization ( 5 ) , molecular association, regulation ( 1 , 2 ) , protein stabilization ( 2 )
Effects of modification on biological processes:	signaling pathway regulation ( 2 ) , transcription, induced ( 2 , 5 )
Induce interaction with:	BTRC (human) ( 2 ) , PIN1 (human) ( 1 )

References
1	Saeidi S, et al. (2022) Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 directly binds and stabilizes Nrf2 in breast cancer. FASEB J 36, e22068 34918396 Curated Info
2	Matzinger M, et al. (2020) AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Redox Biol 29, 101393 31805502 Curated Info
3	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
4	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
5	Sun Z, Huang Z, Zhang DD (2009) Phosphorylation of Nrf2 at multiple sites by MAP kinases has a limited contribution in modulating the Nrf2-dependent antioxidant response. PLoS One 4, e6588 19668370 Curated Info