Tyr801
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Home > Phosphorylation Site Page: > Tyr801  -  VEGFR2 (human)

Site Information
GGELKTGyLSIVMDP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450277

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 4 , 5 , 6 ) , phospho-antibody ( 3 , 4 , 5 , 6 ) , western blotting ( 3 , 4 , 5 , 6 )
Disease tissue studied:
lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 ) , BAEC (endothelial) ( 5 ) , BAE ( 6 ) , COS (fibroblast) ( 5 ) , HEK293T (epithelial) ( 6 ) , HES-3 ('stem, embryonic') ( 2 ) , HUVEC (endothelial) ( 3 , 4 , 6 ) , lung ( 1 )

Upstream Regulation
Putative upstream phosphatases:
PTPRJ (human) ( 4 )
Phosphatases, in vitro:
PTPRJ (human) ( 4 )
Treatments:
VEGF ( 3 , 5 )

Downstream Regulation
Effects of modification on VEGFR2:
activity, induced ( 5 ) , molecular association, regulation ( 5 ) , phosphorylation ( 5 , 6 )
Induce interaction with:
PIK3R1 (human) ( 5 )

References 

1

Ren H (2011) CST Curation Set: 11781; Year: 2011; Biosample/Treatment: cell line, RP98/untreated; Disease: non-small cell lung adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

3

Chen TT, et al. (2010) Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188, 595-609
20176926   Curated Info

4

Chabot C, et al. (2009) New role for the protein tyrosine phosphatase DEP-1 in Akt activation and endothelial cell survival. Mol Cell Biol 29, 241-53
18936167   Curated Info

5

Blanes MG, Oubaha M, Rautureau Y, Gratton JP (2007) Phosphorylation of tyrosine 801 of vascular endothelial growth factor receptor-2 is necessary for Akt-dependent endothelial nitric-oxide synthase activation and nitric oxide release from endothelial cells. J Biol Chem 282, 10660-9
17303569   Curated Info

6

Zhang R, et al. (2003) Etk/Bmx transactivates vascular endothelial growth factor 2 and recruits phosphatidylinositol 3-kinase to mediate the tumor necrosis factor-induced angiogenic pathway. J Biol Chem 278, 51267-76
14532277   Curated Info