Ser376
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Home > Phosphorylation Site Page: > Ser376  -  p53 (human)

Site Information
LkskkGQstsRHkkL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447570

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , electrophoretic mobility shift ( 7 ) , immunoprecipitation ( 7 ) , mutation of modification site ( 2 , 3 , 4 , 6 , 10 ) , phospho-antibody ( 5 , 8 , 9 ) , western blotting ( 5 , 8 , 9 , 10 )
Disease tissue studied:
bone cancer ( 6 , 9 ) , breast cancer ( 9 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
TERT (human) ( 8 )
Kinases, in vitro:
CDK7 (human) ( 11 ) , GSK3B (human) ( 4 ) , PKCA (human) ( 5 , 10 )
Treatments:
development ( 8 ) , ionizing_radiation ( 7 , 9 ) , thapsigargin ( 4 ) , tunicamycin ( 4 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 11 ) , activity, inhibited ( 7 ) , intracellular localization ( 2 , 3 , 4 , 5 ) , molecular association, regulation ( 6 , 7 , 9 , 11 ) , protein degradation ( 3 )
Effects of modification on biological processes:
apoptosis, inhibited ( 4 ) , cell cycle regulation ( 6 ) , chromatin organization, altered ( 5 ) , transcription, induced ( 6 )
Induce interaction with:
14-3-3 gamma (human) ( 6 ) , DNA ( 11 )
Inhibit interaction with:
14-3-3 beta (mouse) ( 7 ) , 14-3-3 zeta (human) ( 9 ) , BLM (human) ( 5 ) , WRN (human) ( 5 )

References 

1

van Dieck J, et al. (2009) Posttranslational modifications affect the interaction of S100 proteins with tumor suppressor p53. J Mol Biol 394, 922-30
19819244   Curated Info

2

Lee MK, Sabapathy K (2007) Phosphorylation at carboxyl-terminal S373 and S375 residues and 14-3-3 binding are not required for mouse p53 function. Neoplasia 9, 690-8
17898864   Curated Info

3

Pluquet O, Qu LK, Baltzis D, Koromilas AE (2005) Endoplasmic reticulum stress accelerates p53 degradation by the cooperative actions of Hdm2 and glycogen synthase kinase 3beta. Mol Cell Biol 25, 9392-405
16227590   Curated Info

4

Qu L, et al. (2004) Endoplasmic reticulum stress induces p53 cytoplasmic localization and prevents p53-dependent apoptosis by a pathway involving glycogen synthase kinase-3beta. Genes Dev 18, 261-77
14744935   Curated Info

5

Yang Q, et al. (2002) The processing of Holliday junctions by BLM and WRN helicases is regulated by p53. J Biol Chem 277, 31980-7
12080066   Curated Info

6

Stavridi ES, Chehab NH, Malikzay A, Halazonetis TD (2001) Substitutions that compromise the ionizing radiation-induced association of p53 with 14-3-3 proteins also compromise the ability of p53 to induce cell cycle arrest. Cancer Res 61, 7030-3
11585729   Curated Info

7

Satyamoorthy K, et al. (2000) Aberrant regulation and function of wild-type p53 in radioresistant melanoma cells. Cell Growth Differ 11, 467-74
11007451   Curated Info

8

Webley K, et al. (2000) Posttranslational modifications of p53 in replicative senescence overlapping but distinct from those induced by DNA damage. Mol Cell Biol 20, 2803-8
10733583   Curated Info

9

Waterman MJ, Stavridi ES, Waterman JL, Halazonetis TD (1998) ATM-dependent activation of p53 involves dephosphorylation and association with 14-3-3 proteins. Nat Genet 19, 175-8
9620776   Curated Info

10

Youmell M, Park SJ, Basu S, Price BD (1998) Regulation of the p53 protein by protein kinase C alpha and protein kinase C zeta. Biochem Biophys Res Commun 245, 514-8
9571186   Curated Info

11

Lu H, Fisher RP, Bailey P, Levine AJ (1997) The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro. Mol Cell Biol 17, 5923-34
9315650   Curated Info