Ser49
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Home > Phosphorylation Site Page: > Ser49  -  eIF2-alpha (human)

Site Information
IEGMILLsELsRRRI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450210

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 ) , mutation of modification site ( 2 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
HRI (human) ( 4 ) , PERK (human) ( 2 ) , PKR (human) ( 3 , 4 )

Downstream Regulation
Effects of modification on eIF2-alpha:
molecular association, regulation ( 3 )
Effects of modification on biological processes:
translation, altered ( 3 )
Induce interaction with:
PERK (human) ( 3 ) , eIF2-beta (human) ( 3 )

References 

1

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

2

Suragani RN, Ghosh S, Ehtesham NZ, Ramaiah KV (2006) Expression and purification of the subunits of human translational initiation factor 2 (eIF2): phosphorylation of eIF2 alpha and beta. Protein Expr Purif 47, 225-33
16289913   Curated Info

3

Suragani RN, Kamindla R, Ehtesham NZ, Ramaiah KV (2005) Interaction of recombinant human eIF2 subunits with eIF2B and eIF2alpha kinases. Biochem Biophys Res Commun 338, 1766-72
16288713   Curated Info

4

Pathak VK, Schindler D, Hershey JW (1988) Generation of a mutant form of protein synthesis initiation factor eIF-2 lacking the site of phosphorylation by eIF-2 kinases. Mol Cell Biol 8, 993-5
3352609   Curated Info