Ser178
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Home > Phosphorylation Site Page: > Ser178  -  p27Kip1 (human)

Site Information
EENVsDGsPNAGsVE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450208

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 , 9 ) , electrophoretic mobility shift ( 9 ) , immunoprecipitation ( 7 ) , mass spectrometry ( 1 , 2 , 3 , 5 , 6 ) , mutation of modification site ( 7 , 8 , 9 ) , phosphoamino acid analysis ( 8 , 9 ) , phosphopeptide mapping ( 9 ) , western blotting ( 7 )
Disease tissue studied:
brain cancer ( 8 ) , astrocytoma ( 8 ) , breast cancer ( 2 ) , breast ductal carcinoma ( 2 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 ) , lung cancer ( 3 ) , non-small cell lung adenocarcinoma ( 3 ) , ovarian cancer ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 7 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 9 ) , breast ( 1 , 2 ) , COS (fibroblast) ( 9 ) , HEK293T (epithelial) ( 9 ) , HeLa (cervical) ( 9 ) , HeLa_Hesp (cervical) ( 6 ) , Jurkat (T lymphocyte) ( 5 ) , lung ( 3 ) , MEF (fibroblast) [PIN1 (human), transfection] ( 7 ) , ovary ( 2 ) , U87MG (glial) ( 8 )

Upstream Regulation
Kinases, in vitro:
ERK2 (human) ( 9 )
Treatments:
LY294002 ( 8 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

4

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

5

Guo A (2011) CST Curation Set: 11984; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP
Curated Info

6

Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12
22067460   Curated Info

7

Zhou W, et al. (2009) Pin1 catalyzes conformational changes of Thr-187 in p27Kip1 and mediates its stability through a polyubiquitination process. J Biol Chem 284, 23980-8
19584057   Curated Info

8

Brandts CH, et al. (2005) Phosphorylation-independent stabilization of p27kip1 by the phosphoinositide 3-kinase pathway in glioblastoma cells. J Biol Chem 280, 2012-9
15542603   Curated Info

9

Ishida N, Kitagawa M, Hatakeyama S, Nakayama K (2000) Phosphorylation at serine 10, a major phosphorylation site of p27(Kip1), increases its protein stability. J Biol Chem 275, 25146-54
10831586   Curated Info