Tyr12
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Home > Phosphorylation Site Page: > Tyr12  -  ACTN1 (human)

Site Information
DsQQtNDyMQPEEDW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450200

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 4 , 5 , 6 ) , mutation of modification site ( 2 , 3 , 7 , 8 ) , phospho-antibody ( 8 ) , western blotting ( 3 )
Disease tissue studied:
bone cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
PAR-4 (human) ( 3 ) , PAR1 (human) ( 3 )
Putative in vivo kinases:
FAK (human) ( 8 )
Kinases, in vitro:
FAK (human) ( 8 )
Treatments:
vanadate ( 8 )

Downstream Regulation
Effects of modification on ACTN1:
molecular association, regulation ( 3 , 7 ) , phosphorylation ( 7 )
Effects of modification on biological processes:
cell motility, altered ( 7 ) , cytoskeletal reorganization ( 2 )
Induce interaction with:
ITGA2B (human) ( 3 ) , Src (human) ( 7 )

References 

1

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

2

Feng Y, et al. (2013) α-actinin1 and 4 tyrosine phosphorylation is critical for stress fiber establishment, maintenance and focal adhesion maturation. Exp Cell Res 319, 1124-35
23454549   Curated Info

3

Tadokoro S, et al. (2011) A potential role for α-actinin in inside-out αIIbβ3 signaling. Blood 117, 250-8
20940419   Curated Info

4

Guo A (2010) CST Curation Set: 9182; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Guo A (2010) CST Curation Set: 9181; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Moritz A (2008) CST Curation Set: 5100; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Zhang Z, Lin SY, Neel BG, Haimovich B (2006) Phosphorylated alpha-actinin and protein-tyrosine phosphatase 1B coregulate the disassembly of the focal adhesion kinase x Src complex and promote cell migration. J Biol Chem 281, 1746-54
16291744   Curated Info

8

Izaguirre G, et al. (2001) The cytoskeletal/non-muscle isoform of alpha-actinin is phosphorylated on its actin-binding domain by the focal adhesion kinase. J Biol Chem 276, 28676-85
11369769   Curated Info