Ser302
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Home > Phosphorylation Site Page: > Ser302  -  IRS1 (rat)

Site Information
TRRSRTEsITATsPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450190

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 8 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 8 )
Relevant cell line - cell type - tissue:
'brain, hypothalamus' ( 4 ) , adipocyte ( 8 ) , BAEC (endothelial) ( 3 ) , C2C12 (myoblast) ( 6 ) , E.coli (bacterial) ( 1 ) , granulosa ( 2 ) , H9c2 (myoblast) ( 6 ) , HEK293T (epithelial) ( 1 ) , L6 (myoblast) ( 1 , 5 ) , liver ( 4 ) , MEF (fibroblast) ( 1 ) , myoblast ( 1 )

Upstream Regulation
Putative in vivo kinases:
ROCK1 (rat) ( 6 )
Treatments:
bradykinin ( 8 ) , IGF-1 ( 1 ) , insulin ( 3 , 4 , 8 ) , leptin ( 4 ) , PF-4708671 ( 1 ) , rapamycin ( 1 ) , serum_withdrawal ( 6 ) , Torin1 ( 1 ) , Y27632 ( 6 )

References 

1

Yoneyama Y, et al. (2018) Serine Phosphorylation by mTORC1 Promotes IRS-1 Degradation through SCFβ-TRCP E3 Ubiquitin Ligase. iScience 5, 1-18
30240640   Curated Info

2

Law NC, White MF, Hunzicker-Dunn ME (2016) G protein-coupled receptors (GPCRs) That Signal via Protein Kinase A (PKA) Cross-talk at Insulin Receptor Substrate 1 (IRS1) to Activate the phosphatidylinositol 3-kinase (PI3K)/AKT Pathway. J Biol Chem 291, 27160-27169
27856640   Curated Info

3

Maeno Y, et al. (2012) Inhibition of Insulin Signaling in Endothelial Cells by Protein Kinase C-induced Phosphorylation of p85 Subunit of Phosphatidylinositol 3-Kinase (PI3K). J Biol Chem 287, 4518-30
22158866   Curated Info

4

Berthou F, et al. (2011) Chronic central leptin infusion differently modulates brain and liver insulin signaling. Mol Cell Endocrinol 337, 89-95
21320568   Curated Info

5

Giraud J, et al. (2007) Phosphorylation of Irs1 at SER-522 inhibits insulin signaling. Mol Endocrinol 21, 2294-302
17579213   Curated Info

6

Lim MJ, et al. (2007) RhoA/Rho kinase blocks muscle differentiation via serine phosphorylation of insulin receptor substrate-1 and -2. Mol Endocrinol 21, 2282-93
17579208   Curated Info

7

Herschkovitz A, et al. (2007) Common inhibitory serine sites phosphorylated by IRS-1 kinases, triggered by insulin and inducers of insulin resistance. J Biol Chem 282, 18018-27
17446166   Curated Info

8

Beard KM, Lu H, Ho K, Fantus IG (2006) Bradykinin augments insulin-stimulated glucose transport in rat adipocytes via endothelial nitric oxide synthase-mediated inhibition of Jun NH2-terminal kinase. Diabetes 55, 2678-87
17003331   Curated Info