Lys154
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Home > Ubiquitylation Site Page: > Lys154  -  p21Cip1 (human)

Site Information
MTDFYHSkRRLIFSK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 9897005

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 3 , 4 ) , immunoprecipitation ( 1 , 4 ) , modification-specific antibody ( 4 ) , mutation of modification site ( 1 , 3 , 4 , 5 ) , western blotting ( 1 , 3 , 4 )
Disease tissue studied:
bone cancer ( 1 , 5 ) , breast cancer ( 4 ) , melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 4 ) , A375 (melanocyte) ( 3 ) , MCF-7 (breast cell) ( 4 ) , U2OS (bone cell) ( 1 , 5 )

Upstream Regulation
Treatments:
311 ( 4 ) , IL-1a ( 3 )

Downstream Regulation
Effects of modification on p21Cip1:
protein degradation ( 1 , 4 , 5 ) , ubiquitination ( 5 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell cycle regulation ( 1 ) , cell growth, induced ( 1 )

References 

1

Chen Z, et al. (2017) CRL4BDCAF11 E3 ligase targets p21 for degradation to control cell cycle progression in human osteosarcoma cells. Sci Rep 7, 1175
28446751   Curated Info

2

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

3

Arakawa T, et al. (2010) IL-1-induced ERK1/2 activation up-regulates p21(Waf1/Cip1) protein by inhibition of degradation via ubiquitin-independent pathway in human melanoma cells A375. Biochem Biophys Res Commun 392, 369-372
20074553   Curated Info

4

Fu D, Richardson DR (2007) Iron chelation and regulation of the cell cycle: 2 mechanisms of posttranscriptional regulation of the universal cyclin-dependent kinase inhibitor p21CIP1/WAF1 by iron depletion. Blood 110, 752-61
17429006   Curated Info

5

Bendjennat M, et al. (2003) UV irradiation triggers ubiquitin-dependent degradation of p21(WAF1) to promote DNA repair. Cell 114, 599-610
13678583   Curated Info

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