Ser985
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Home > Phosphorylation Site Page: > Ser985  -  Met (human)

Site Information
PHLDRLVsARsVsPt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450188

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , immunoprecipitation ( 2 , 4 ) , mass spectrometry ( 1 ) , mutation of modification site ( 2 , 6 ) , phospho-antibody ( 2 , 3 , 4 , 5 ) , phosphoamino acid analysis ( 6 ) , phosphopeptide mapping ( 6 ) , western blotting ( 2 , 3 , 4 , 5 )
Disease tissue studied:
gastric cancer ( 2 ) , gastric carcinoma ( 2 ) , lung cancer ( 5 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 5 ) , AGS (gastric) ( 2 ) , COS (fibroblast) ( 6 ) , COS7 (fibroblast) ( 2 ) , HeLa (cervical) ( 1 ) , hepatocyte-liver ( 4 ) , HGC-27 (gastric) ( 2 ) , HLMVECs ( 3 ) , liver ( 4 )

Upstream Regulation
Putative in vivo kinases:
PKCD (human) ( 5 ) , PKCE (human) ( 5 ) , PKG2 (human) ( 2 )
Kinases, in vitro:
PKCA (human) ( 6 )
Putative upstream phosphatases:
PPP2CB (human) ( 5 )
Treatments:
A23187 ( 6 ) , bisindolylmaleimide ( 5 ) , CCL4 ( 4 ) , H2O2 ( 5 ) , HGF ( 3 , 4 , 5 ) , LY294002 ( 3 ) , NaF ( 4 , 5 ) , phorbol_ester ( 4 , 5 , 6 ) , Su11274 ( 3 )

Downstream Regulation
Effects of modification on Met:
enzymatic activity, inhibited ( 2 , 6 ) , intracellular localization ( 4 )
Effects of modification on biological processes:
cell motility, induced ( 3 ) , signaling pathway regulation ( 3 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Wu Y, et al. (2016) PKG II reverses HGF-triggered cellular activities by phosphorylating serine 985 of c-Met in gastric cancer cells. Oncotarget 7, 34190-200
27147579   Curated Info

3

Usatyuk PV, et al. (2014) Role of c-Met/phosphatidylinositol 3-kinase (PI3k)/Akt signaling in hepatocyte growth factor (HGF)-mediated lamellipodia formation, reactive oxygen species (ROS) generation, and motility of lung endothelial cells. J Biol Chem 289, 13476-91
24634221   Curated Info

4

Nakayama M, et al. (2013) Met/HGF receptor activation is regulated by juxtamembrane Ser985 phosphorylation in hepatocytes. Cytokine 62, 446-52
23618918   Curated Info

5

Hashigasako A, et al. (2004) Bi-directional regulation of Ser-985 phosphorylation of c-met via protein kinase C and protein phosphatase 2A involves c-Met activation and cellular responsiveness to hepatocyte growth factor. J Biol Chem 279, 26445-52
15075332   Curated Info

6

Gandino L, et al. (1994) Phosphorylation of serine 985 negatively regulates the hepatocyte growth factor receptor kinase. J Biol Chem 269, 1815-20
8294430   Curated Info