Ser273

Site Information
ILPttAksPLGNIRs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3213432

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 2 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 8 ) , 32Dcl3 (myeloid) ( 8 ) , HeLa (cervical) ( 6 ) , lung ( 7 ) , macrophage-peritoneum ( 4 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 5 ) , RAW 264.7 (macrophage) ( 3 ) , spleen ( 7 ) , testis ( 7 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 )

Relevant cell line - cell type - tissue:

'3T3-L1, differentiated' (adipocyte) ( 2 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 8 ) , 32Dcl3 (myeloid) ( 8 ) , HeLa (cervical) ( 6 ) , lung ( 7 ) , macrophage-peritoneum ( 4 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 5 ) , RAW 264.7 (macrophage) ( 3 ) , spleen ( 7 ) , testis ( 7 )

Upstream Regulation
Regulatory protein:	FLT3 (mouse) ( 8 )

References
1	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
2	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
3	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
4	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
5	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
6	Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12 22067460 Curated Info
7	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
8	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info