Ser1303
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Home > Phosphorylation Site Page: > Ser1303  -  NMDAR2B (rat)

Site Information
NKLRRQHsyDtFVDL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450171

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 49 ) , immunoprecipitation ( 15 ) , mass spectrometry ( 7 , 12 , 15 , 16 , 20 , 21 , 22 , 23 , 24 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ) , microscopy-colocalization with upstream kinase ( 48 ) , mutation of modification site ( 48 ) , peptide sequencing ( 49 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 13 , 14 , 17 , 18 , 19 , 45 , 46 , 49 ) , phosphoamino acid analysis ( 49 ) , phosphopeptide mapping ( 12 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 13 , 14 , 17 , 18 , 19 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 16 ) , 'brain, cerebellum' ( 16 ) , 'brain, cerebral cortex' ( 5 , 14 , 16 ) , 'brain, hippocampus' ( 5 , 6 , 14 ) , 'brain, hypothalamus' ( 5 ) , 'brain, nucleus accumbens' ( 14 ) , 'brain, striatum' ( 6 , 14 , 46 ) , 'brain, visual cortex' ( 19 ) , 'neuron, cortical'-brain ( 2 , 6 ) , 'neuron, hippocampal'-brain ( 3 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 8 ) , 293 (epithelial) ( 48 ) , brain ( 7 , 9 , 13 , 18 , 20 , 21 , 22 , 23 , 24 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ) , esophagus ( 17 ) , neuron-'brain, cerebral cortex' ( 10 ) , neuron-'brain, hippocampus' ( 4 , 15 , 49 ) , neuron-spinal cord ( 11 ) , neuron:synaptosome-brain ( 12 ) , retina ( 19 ) , spinal cord ( 45 )

Upstream Regulation
Regulatory protein:
5-HT(2A) (mouse) ( 9 ) , DRD2 (human) ( 46 ) , IL-1RA (human) ( 3 )
Putative in vivo kinases:
CAMK2A (rat) ( 17 , 46 ) , DAPK1 (rat) ( 2 , 7 ) , PKCD (human) ( 3 )
Kinases, in vitro:
CAMK2A (human) ( 46 ) , CAMK2A (rat) ( 47 , 48 , 49 )
Putative upstream phosphatases:
PPP1CA (rat) ( 13 )
Phosphatases, in vitro:
PPP1CA (human) ( 13 , 25 ) , PPP1CB (human) ( 13 ) , PPP1CC (human) ( 13 ) , PPP2CA (human) ( 25 )
Treatments:
1-methoxyoctadecan-1-ol ( 10 ) , A23187 ( 48 ) , BDE 209 ( 4 ) , bisindolylmaleimide ( 13 ) , calcineurin ( 2 , 13 ) , chronic unpredictable stress ( 7 ) , CK59 ( 2 ) , cocaine ( 46 ) , DAPK_inhibitor ( 2 ) , dark_adaptation ( 19 ) , deoxygenation ( 2 ) , DOI ( 9 ) , EGTA ( 49 ) , ethanol ( 45 ) , eticlopride ( 46 ) , ginsenoside ( 2 ) , glutamic acid ( 10 ) , Go_6983 ( 2 ) , I-2 ( 13 ) , ischemia/reperfusion ( 12 ) , ketamine ( 6 ) , KN-92 ( 46 ) , KN-93 ( 17 , 46 ) , light ( 19 ) , memantine ( 11 ) , nicotine ( 14 ) , NMDA ( 2 ) , novel object recognition test ( 8 ) , okadaic_acid ( 13 , 49 ) , PP2 ( 3 ) , quinpirole ( 46 ) , rottlerin ( 3 ) , SCH_23390 ( 46 ) , SKF81297 ( 46 ) , stress ( 8 )

Downstream Regulation
Effects of modification on NMDAR2B:
intracellular localization ( 48 ) , molecular association, regulation ( 48 ) , phosphorylation ( 3 )
Effects of modification on biological processes:
signaling pathway regulation ( 2 )
Inhibit interaction with:
CAMK2A (human) ( 48 )

Disease / Diagnostics Relevance
Relevant diseases:
hypertension ( 5 )

References 

1

Lee GS, Zhang J, Wu Y, Zhou Y (2021) 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PLoS One 16, e0261791
34962957   Curated Info

2

Zhang C, et al. (2020) Protopanaxadiol ginsenoside Rd protects against NMDA receptor-mediated excitotoxicity by attenuating calcineurin-regulated DAPK1 activity. Sci Rep 10, 8078
32415270   Curated Info

3

Taoro-Gonzalez L, Arenas YM, Cabrera-Pastor A, Felipo V (2018) Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms. J Neuroinflammation 15, 36
29422059   Curated Info

4

Xiong L, et al. (2018) Effect of decabrominated diphenyl ether exposure on spatial learning and memory, the expression and phosphorylation of hippocampal glutamate receptor subunits in adult Sprague-Dawley rats. J Toxicol Sci 43, 645-657
30404998   Curated Info

5

Li DP, Zhou JJ, Zhang J, Pan HL (2017) CaMKII Regulates Synaptic NMDA Receptor Activity of Hypothalamic Presympathetic Neurons and Sympathetic Outflow in Hypertension. J Neurosci 37, 10690-10699
28972129   Curated Info

6

Caffino L, et al. (2017) Ketamine Self-Administration Elevates ╬▒CaMKII Autophosphorylation in Mood and Reward-Related Brain Regions in Rats. Mol Neurobiol
28948570   Curated Info

7

Li SX, et al. (2017) Uncoupling DAPK1 from NMDA receptor GluN2B subunit exerts rapid antidepressant-like effects. Mol Psychiatry
28439098   Curated Info

8

Calabrese F, et al. (2017) Chronic Mild Stress-Induced Alterations of Local Protein Synthesis: A Role for Cognitive Impairment. ACS Chem Neurosci 8, 817-825
28094500   Curated Info

9

Barre A, et al. (2016) Presynaptic serotonin 2A receptors modulate thalamocortical plasticity and associative learning. Proc Natl Acad Sci U S A 113, E1382-91
26903620   Curated Info

10

Jang JY, et al. (2014) Neuroprotective effects of a novel single compound 1-methoxyoctadecan-1-ol isolated from uncaria sinensis in primary cortical neurons and a photothrombotic ischemia model. PLoS One 9, e85322
24416390   Curated Info

11

Morel V, et al. (2013) Memantine, a promising drug for the prevention of neuropathic pain in rat. Eur J Pharmacol 721, 382-90
23810967   Curated Info

12

Zhang F, et al. (2013) Phosphorylation and assembly of glutamate receptors after brain ischemia. Stroke 44, 170-6
23212166   Curated Info

13

Prabhu Ramya R, Suma Priya S, Mayadevi M, Omkumar RV (2012) Regulation of phosphorylation at Ser(1303) of GluN2B receptor in the postsynaptic density. Neurochem Int 61, 981-5
22982438   Curated Info

14

Nakajima A, et al. (2012) Repeated treatment with nicotine induces phosphorylation of NMDA receptor NR2B subunit in the brain regions involved in behavioral sensitization. Neurosci Lett 524, 133-8
22819974   Curated Info

15

Ghafari M, et al. (2012) Mass Spectrometrical Identification of Hippocampal NMDA Receptor Subunits NR1, NR2A-D and Five Novel Phosphorylation Sites on NR2A and NR2B. J Proteome Res 11, 1891-6
22335236   Curated Info

16

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

17

Banerjee B, et al. (2011) Neuronal plasticity in the cingulate cortex of rats following esophageal acid exposure in early life. Gastroenterology 141, 544-52
21616075   Curated Info

18

Castillo C, et al. (2011) The N-methyl-D-aspartate-evoked cytoplasmic calcium increase in adult rat dorsal root ganglion neuronal somata was potentiated by substance P pretreatment in a protein kinase C-dependent manner. Neuroscience 177, 308-20
21215796   Curated Info

19

Giannakopoulos M, Kouvelas ED, Mitsacos A (2010) Experience-dependent regulation of NMDA receptor subunit composition and phosphorylation in the retina and visual cortex. Invest Ophthalmol Vis Sci 51, 1817-22
19850826   Curated Info

20

Hu Y (2010) CST Curation Set: 8874; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

21

Hu Y (2010) CST Curation Set: 8921; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

22

Hu Y (2010) CST Curation Set: 8875; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

23

Hu Y (2010) CST Curation Set: 8881; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

24

Hu Y (2010) CST Curation Set: 8880; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

25

Raveendran R, et al. (2009) Phosphorylation status of the NR2B subunit of NMDA receptor regulates its interaction with calcium/calmodulin-dependent protein kinase II. J Neurochem 110, 92-105
19453375   Curated Info

26

Guo A (2009) CST Curation Set: 5871; Year: 2009; Biosample/Treatment: tissue, brain/No seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

27

Guo A (2009) CST Curation Set: 5870; Year: 2009; Biosample/Treatment: tissue, brain/No seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

28

Guo A (2009) CST Curation Set: 5867; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

29

Guo A (2009) CST Curation Set: 5868; Year: 2009; Biosample/Treatment: tissue, brain/No seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

30

Guo A (2009) CST Curation Set: 5869; Year: 2009; Biosample/Treatment: tissue, brain/No seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

31

Guo A (2009) CST Curation Set: 5862; Year: 2009; Biosample/Treatment: tissue, brain/sham; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

32

Guo A (2009) CST Curation Set: 5863; Year: 2009; Biosample/Treatment: tissue, brain/sham; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

33

Guo A (2009) CST Curation Set: 5864; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

34

Guo A (2009) CST Curation Set: 5865; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

35

Guo A (2009) CST Curation Set: 5866; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

36

Guo A (2009) CST Curation Set: 5861; Year: 2009; Biosample/Treatment: tissue, brain/sham; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

37

Guo A (2009) CST Curation Set: 5860; Year: 2009; Biosample/Treatment: tissue, brain/sham; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

38

Guo A (2008) CST Curation Set: 5216; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

39

Guo A (2008) CST Curation Set: 5217; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

40

Guo A (2008) CST Curation Set: 5218; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

41

Guo A (2008) CST Curation Set: 5219; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

42

Guo A (2008) CST Curation Set: 5220; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

43

Guo A (2008) CST Curation Set: 5221; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

44

Guo A (2008) CST Curation Set: 5222; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

45

Narita M, Miyoshi K, Narita M, Suzuki T (2007) Changes in function of NMDA receptor NR2B subunit in spinal cord of rats with neuropathy following chronic ethanol consumption. Life Sci 80, 852-9
17156796   Curated Info

46

Liu XY, et al. (2006) Modulation of D2R-NR2B interactions in response to cocaine. Neuron 52, 897-909
17145509   Curated Info

47

Mayadevi M, Praseeda M, Kumar KS, Omkumar RV (2002) Sequence determinants on the NR2A and NR2B subunits of NMDA receptor responsible for specificity of phosphorylation by CaMKII. Biochim Biophys Acta 1598, 40-5
12147342   Curated Info

48

Strack S, McNeill RB, Colbran RJ (2000) Mechanism and regulation of calcium/calmodulin-dependent protein kinase II targeting to the NR2B subunit of the N-methyl-D-aspartate receptor. J Biol Chem 275, 23798-806
10764765   Curated Info

49

Omkumar RV, et al. (1996) Identification of a phosphorylation site for calcium/calmodulindependent protein kinase II in the NR2B subunit of the N-methyl-D-aspartate receptor. J Biol Chem 271, 31670-8
8940188   Curated Info