Ser272
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Home > Phosphorylation Site Page: > Ser272  -  5-LO (human)

Site Information
CsLERQLsLEQEVQQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450169

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 4 ) , mass spectrometry (in vitro) ( 4 ) , mutation of modification site ( 5 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 )
Disease tissue studied:
leukemia ( 4 ) , acute myelogenous leukemia ( 4 ) , lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 6 , 9 ) , 3T3 (fibroblast) ( 5 ) , Calu 6 (pulmonary) ( 1 ) , COS (fibroblast) ( 5 ) , HEK293T (epithelial) ( 2 ) , HeLa (cervical) ( 7 , 8 , 9 ) , Mono Mac 6 (myeloid) ( 4 , 9 ) , neutrophil-blood ( 9 )

Upstream Regulation
Kinases, in vitro:
CAMK2A (human) ( 4 , 9 ) , MAPKAPK2 (human) ( 4 , 9 , 10 ) , PKACA (human) ( 4 , 9 )
Treatments:
A23187 ( 9 ) , apocynin ( 1 ) , fMLP ( 9 ) , KN-93 ( 5 ) , SB203580 ( 1 , 5 ) , WKYMVm ( 1 ) , WRW4 ( 1 )

Downstream Regulation
Effects of modification on 5-LO:
enzymatic activity, induced ( 6 , 7 , 9 ) , intracellular localization ( 2 , 3 , 5 , 6 , 9 )

References 

1

Pecchillo Cimmino T, et al. (2024) Formyl Peptide Receptor 2-Dependent cPLA2 and 5-LOX Activation Requires a Functional NADPH Oxidase. Antioxidants (Basel) 13
38397818   Curated Info

2

Ball AK, et al. (2017) Characterization and cellular localization of human 5-lipoxygenase and its protein isoforms 5-LOΔ13, 5-LOΔ4 and 5-LOp12. Biochim Biophys Acta 1862, 561-571
28257804   Curated Info

3

Allain EP, Boudreau LH, Flamand N, Surette ME (2015) The Intracellular Localisation and Phosphorylation Profile of the Human 5-Lipoxygenase Δ13 Isoform Differs from That of Its Full Length Counterpart. PLoS One 10, e0132607
26173130   Curated Info

4

Markoutsa S, et al. (2014) Analysis of 5-lipoxygenase phosphorylation on molecular level by MALDI-MS. FEBS J 281, 1931-47
24860872   Curated Info

5

Flamand N, Luo M, Peters-Golden M, Brock TG (2009) Phosphorylation of serine 271 on 5-lipoxygenase and its role in nuclear export. J Biol Chem 284, 306-13
18978352   Curated Info

6

Hanaka H, Shimizu T, Izumi T (2005) Stress-induced nuclear export of 5-lipoxygenase. Biochem Biophys Res Commun 338, 111-6
16165096   Curated Info

7

Fischer L, et al. (2003) Phosphorylation- and stimulus-dependent inhibition of cellular 5-lipoxygenase activity by nonredox-type inhibitors. FASEB J 17, 949-51
12670876   Curated Info

8

Werz O, et al. (2002) Extracellular signal-regulated kinases phosphorylate 5-lipoxygenase and stimulate 5-lipoxygenase product formation in leukocytes. FASEB J 16, 1441-3
12205041   Curated Info

9

Werz O, Szellas D, Steinhilber D, Rådmark O (2002) Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2). J Biol Chem 277, 14793-800
11844797   Curated Info

10

Werz O, Klemm J, Samuelsson B, Rådmark O (2000) 5-lipoxygenase is phosphorylated by p38 kinase-dependent MAPKAP kinases. Proc Natl Acad Sci U S A 97, 5261-6
10779545   Curated Info

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