Ser870
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Home > Phosphorylation Site Page: > Ser870  -  NFkB-p100 (human)

Site Information
kEDsAyGsQsVEQEA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450167

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 ) , mass spectrometry ( 2 , 5 ) , mutation of modification site ( 4 , 6 , 7 ) , phospho-antibody ( 1 , 3 , 4 ) , western blotting ( 1 , 3 , 4 )
Disease tissue studied:
bone cancer ( 1 ) , colorectal cancer ( 4 ) , colorectal carcinoma ( 4 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 ) , non-small cell squamous cell lung carcinoma ( 5 ) , lymphoma ( 4 ) , B cell lymphoma ( 4 ) , prostate cancer ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 6 , 7 ) , DU 145 (prostate cell) ( 1 ) , HCAEC (endothelial) ( 1 ) , HCT116 (intestinal) ( 4 ) , HeLa (cervical) ( 2 ) , M12 (B lymphocyte) ( 4 ) , NCI-H1703 (squamous) ( 5 ) , U2OS (bone cell) ( 1 )

Upstream Regulation
Regulatory protein:
IKKB (human) ( 1 ) , Nik (human) ( 4 )
Putative in vivo kinases:
IKKA (human) ( 1 )
Kinases, in vitro:
IKKA (human) ( 4 ) , Nik (human) ( 7 )
Treatments:
antibody ( 4 ) , CW15337 ( 1 ) , GSK-3_inhibitor_IX ( 3 ) , IKK2-X1 ( 1 ) , IL-1b ( 1 ) , Lymphotoxin LTA1B2 ( 1 ) , siRNA ( 1 ) , TNF ( 1 )

Downstream Regulation
Effects of modification on NFkB-p100:
molecular association, regulation ( 3 , 6 ) , protein processing ( 7 )
Induce interaction with:
BTRC (human) ( 3 ) , IKKA (human) ( 6 )

References 

1

McIntosh K, et al. (2023) IL-1β stimulates a novel, IKKα -dependent, NIK -independent activation of non-canonical NFκB signalling. Cell Signal 107, 110684
37080443   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Busino L, et al. (2012) Fbxw7α- and GSK3-mediated degradation of p100 is a pro-survival mechanism in multiple myeloma. Nat Cell Biol 14, 375-85
22388891   Curated Info

4

Vatsyayan J, Qing G, Xiao G, Hu J (2008) SUMO1 modification of NF-kappaB2/p100 is essential for stimuli-induced p100 phosphorylation and processing. EMBO Rep 9, 885-90
18617892   Curated Info

5

Moritz A (2007) CST Curation Set: 3535; Year: 2007; Biosample/Treatment: cell line, NCI-H1703/Gleevec; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

6

Qu Z, Qing G, Rabson A, Xiao G (2004) Tax deregulation of NF-kappaB2 p100 processing involves both beta-TrCP-dependent and -independent mechanisms. J Biol Chem 279, 44563-72
15310758   Curated Info

7

Xiao G, Harhaj EW, Sun SC (2001) NF-kappaB-inducing kinase regulates the processing of NF-kappaB2 p100. Mol Cell 7, 401-9
11239468   Curated Info

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