Thr577
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Home > Phosphorylation Site Page: > Thr577  -  RSK2 (mouse)

Site Information
AENGLLMtPCYtANF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450152

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 9 ) , phospho-antibody ( 8 , 9 ) , western blotting ( 8 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 ) , 'brain, embryonic' ( 5 ) , 3T3 (fibroblast) ( 8 ) , brain ( 6 ) , COS (fibroblast) ( 9 ) , dendritic cell ( 8 ) , dendritic cell [MAPKAPK2 (mouse), homozygous knockout] ( 8 ) , dendritic cell [MSK1 (mouse), homozygous knockout] ( 8 ) , liver ( 7 ) , macrophage-peritoneum ( 4 ) , RAW 264.7 (macrophage) ( 2 )

Upstream Regulation
Regulatory protein:
MAPKAPK2 (mouse) ( 8 ) , MAPKAPK3 (mouse) ( 8 )
Kinases, in vitro:
ERK2 (human) ( 8 ) , PDK1 (human) ( 8 ) , PDK1 (mouse) ( 9 )
Treatments:
BIRB-0796 ( 8 ) , EGF ( 9 ) , IL-33 ( 2 ) , LPS ( 8 ) , PD184352 ( 8 ) , SB203580 ( 8 )

Downstream Regulation
Effects of modification on RSK2:
enzymatic activity, induced ( 9 )

References 

1

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

2

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

5

Guo A (2011) CST Curation Set: 12476; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

6

Guo A (2011) CST Curation Set: 12477; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

7

Guo A (2011) CST Curation Set: 12478; Year: 2011; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

8

Zaru R, et al. (2007) The MAPK-activated kinase Rsk controls an acute Toll-like receptor signaling response in dendritic cells and is activated through two distinct pathways. Nat Immunol 8, 1227-35
17906627   Curated Info

9

Jensen CJ, et al. (1999) 90-kDa ribosomal S6 kinase is phosphorylated and activated by 3-phosphoinositide-dependent protein kinase-1. J Biol Chem 274, 27168-76
10480933   Curated Info