Ser503
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Home > Phosphorylation Site Page: > Ser503  -  OTUD5 (mouse)

Site Information
AGGDRATsPLVSLYP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483402

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 1 ) , mass spectrometry ( 2 , 4 , 6 , 7 , 8 , 9 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
anthrax infection ( 8 ) , breast cancer ( 1 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 2 ) , heart ( 9 ) , HEK293T (epithelial) ( 1 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 4 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 4 ) , HL-1 (myocyte) ( 4 ) , HT-29 (intestinal) ( 1 ) , kidney ( 9 ) , lung ( 9 ) , macrophage-peritoneum ( 6 ) , MCF-7 (breast cell) ( 1 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 7 ) , spleen ( 8 , 9 )

Upstream Regulation
Regulatory protein:
TSC1 (human) ( 1 )
Putative in vivo kinases:
mTOR (mouse) ( 1 )
Kinases, in vitro:
mTOR (mouse) ( 1 )
Treatments:
amino_acid_starvation ( 1 ) , amino_acids ( 1 ) , bortezomib ( 1 ) , glucose ( 1 ) , rapamycin ( 1 ) , starvation ( 1 )

Downstream Regulation
Effects of modification on OTUD5:
enzymatic activity, induced ( 1 ) , molecular association, regulation ( 1 ) , phosphorylation ( 1 ) , protein degradation ( 1 ) , protein stabilization ( 1 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
autophagy, inhibited ( 1 ) , cell cycle regulation ( 1 ) , cell growth, induced ( 1 ) , signaling pathway regulation ( 1 ) , transcription, altered ( 1 )
Inhibit interaction with:
BTRC (human) ( 1 )

References 

1

Cho JH, et al. (2020) Deubiquitinase OTUD5 is a positive regulator of mTORC1 and mTORC2 signaling pathways. Cell Death Differ
33110214   Curated Info

2

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia 32
28852199   Curated Info

3

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

4

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

7

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

8

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

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