Ser235

Site Information
KsIsERLsVLKGAKP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450116

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 3 , 5 , 6 ) , mass spectrometry (in vitro) ( 4 ) , mutation of modification site ( 1 , 2 )
Relevant cell line - cell type - tissue:	lens ( 3 , 5 , 6 ) , oocyte ( 2 ) , Pichia pastoris (yeast cell) ( 1 )

Downstream Regulation
Effects of modification on AQP0:	activity, induced ( 1 ) , molecular association, regulation ( 1 , 2 , 4 ) , protein conformation ( 2 )
Inhibit interaction with:	Calmodulin (cow) ( 4 ) , Calmodulin (human) ( 1 , 2 )

References
1	Kreida S, et al. (2023) The role of phosphorylation in calmodulin-mediated gating of human AQP0. Biochem J 38032258 Curated Info
2	Fields JB, et al. (2017) Calmodulin Gates Aquaporin 0 Permeability through a Positively Charged Cytoplasmic Loop. J Biol Chem 292, 185-195 27660387 Curated Info
3	Gutierrez DB, et al. (2016) Spatial distributions of phosphorylated membrane proteins aquaporin 0 and MP20 across young and aged human lenses. Exp Eye Res 149, 59-65 27339748 Curated Info
4	Rose KM, et al. (2008) Aquaporin 0-calmodulin interaction and the effect of aquaporin 0 phosphorylation. Biochemistry 47, 339-47 18081321 Curated Info
5	Ball LE, Garland DL, Crouch RK, Schey KL (2004) Post-translational modifications of aquaporin 0 (AQP0) in the normal human lens: spatial and temporal occurrence. Biochemistry 43, 9856-65 15274640 Curated Info
6	Schey KL, Little M, Fowler JG, Crouch RK (2000) Characterization of human lens major intrinsic protein structure. Invest Ophthalmol Vis Sci 41, 175-82 10634618 Curated Info