Ser15
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Home > Phosphorylation Site Page: > Ser15  -  p53 (rat)

Site Information
MsIELPLsQEtFsCL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447490

In vivo Characterization
Methods used to characterize site in vivo:
phospho-antibody ( 2 , 3 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , western blotting ( 2 , 3 , 6 , 7 , 8 , 9 , 11 )
Disease tissue studied:
brain cancer ( 8 ) , glioma ( 8 ) , leukemia ( 6 ) , chronic myelogenous leukemia ( 6 ) , pancreatic cancer ( 7 ) , pancreatic carcinoma ( 7 )
Relevant cell line - cell type - tissue:
'muscle, smooth'-thoracic aorta ( 11 ) , 'neuron, cortical' ( 2 ) , 'neuron, cortical'-brain ( 10 ) , C6 (glial) ( 8 ) , INS-1 (pancreatic) ( 7 ) , K562 (erythroid) ( 6 ) , myocyte-heart ( 9 , 12 ) , neuron-'brain, cerebral cortex' ( 2 ) , retinal-retina ( 3 )

Upstream Regulation
Regulatory protein:
CCNA1 (rat) ( 6 ) , JNK1 (rat) ( 10 ) , PPP1R13L (mouse) ( 3 )
Putative in vivo kinases:
ATM (human) ( 5 )
Treatments:
acetominophen ( 8 ) , aminoguanidine ( 9 ) , angiotensin_2 ( 9 ) , aphidicolin ( 11 ) , axotomy ( 3 ) , BEL ( 7 ) , benzo(a)pyrene ( 2 ) , beta-amyloid_40 ( 10 ) , caffeine ( 6 , 7 ) , CGK733 ( 6 ) , DPI ( 9 ) , etoposide ( 8 ) , fluorouracil ( 8 ) , glucose ( 12 ) , H2O2 ( 5 ) , ionizing_radiation ( 11 ) , KU-55933 ( 5 ) , olomoucine ( 6 ) , resveratrol ( 11 ) , seliciclib ( 2 ) , serum ( 11 )

Downstream Regulation
Effects of modification on p53:
protein stabilization ( 10 )
Effects of modification on biological processes:
apoptosis, induced ( 2 , 10 )

References 

1

Wu WY, et al. (2022) SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. Redox Biol 52, 102303
35390676   Curated Info

2

Nie J, et al. (2021) Phosphorylation of p53 by Cdk5 contributes to benzo[a]pyrene-induced neuronal apoptosis. Environ Toxicol
34529316   Curated Info

3

Wilson AM, et al. (2014) Inhibitor of Apoptosis-Stimulating Protein of p53 (iASPP) Is Required for Neuronal Survival after Axonal Injury. PLoS One 9, e94175
24714389   Curated Info

4

Gogna R, et al. (2013) p53's choice of myocardial death or survival: Oxygen protects infarct myocardium by recruiting p53 on NOS3 promoter through regulation of p53-Lys(118) acetylation. EMBO Mol Med 5, 1662-83
24096875   Curated Info

5

Pizarro JG, et al. (2010) ATM is involved in cell-cycle control through the regulation of retinoblastoma protein phosphorylation. J Cell Biochem 110, 210-8
20213763   Curated Info

6

Restle A, et al. (2008) Dissecting the role of p53 phosphorylation in homologous recombination provides new clues for gain-of-function mutants. Nucleic Acids Res 36, 5362-75
18697815   Curated Info

7

Zhang XH, Zhao C, Ma ZA (2007) The increase of cell-membranous phosphatidylcholines containing polyunsaturated fatty acid residues induces phosphorylation of p53 through activation of ATR. J Cell Sci 120, 4134-43
18032786   Curated Info

8

Lee YS, et al. (2006) Ubiquitin-dependent degradation of p53 protein despite phosphorylation at its N terminus by acetaminophen. J Pharmacol Exp Ther 317, 202-8
16330492   Curated Info

9

Grishko V, et al. (2003) Apoptotic cascade initiated by angiotensin II in neonatal cardiomyocytes: role of DNA damage. Am J Physiol Heart Circ Physiol 285, H2364-72
12919932   Curated Info

10

Fogarty MP, Downer EJ, Campbell V (2003) A role for c-Jun N-terminal kinase 1 (JNK1), but not JNK2, in the beta-amyloid-mediated stabilization of protein p53 and induction of the apoptotic cascade in cultured cortical neurons. Biochem J 371, 789-98
12534344   Curated Info

11

Haider UG, et al. (2003) Resveratrol increases serine15-phosphorylated but transcriptionally impaired p53 and induces a reversible DNA replication block in serum-activated vascular smooth muscle cells. Mol Pharmacol 63, 925-32
12644594   Curated Info

12

Fiordaliso F, et al. (2001) Hyperglycemia activates p53 and p53-regulated genes leading to myocyte cell death. Diabetes 50, 2363-75
11574421   Curated Info