Ser283

Site Information
REYQEFssPEtsLPY SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450113

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 ) , peptide sequencing ( 4 ) , phosphoamino acid analysis ( 4 )
Relevant cell line - cell type - tissue:	32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 3 ) , 32Dcl3 (myeloid) ( 3 ) , COS (fibroblast) ( 4 ) , HEK293T (epithelial) ( 4 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 1 ) , spleen ( 2 )

Upstream Regulation
Regulatory protein:	FLT3 (mouse) ( 3 )
Kinases, in vitro:	CDK2 (mouse) ( 4 )

References
1	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
2	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
3	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
4	Johnson TK, Schweppe RE, Septer J, Lewis RE (1999) Phosphorylation of B-Myb regulates its transactivation potential and DNA binding. J Biol Chem 274, 36741-9 10593981 Curated Info