Ser181
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Home > Phosphorylation Site Page: > Ser181  -  IKKB (mouse)

Site Information
DQGsLCTsFVGTLQy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447489

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 9 ) , phospho-antibody ( 1 , 2 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ) , western blotting ( 1 , 2 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 18 )
Disease tissue studied:
breast cancer ( 11 ) , diabetes mellitus ( 11 ) , type 2 diabetes ( 11 )
Relevant cell line - cell type - tissue:
'neuron, superior cervical ganglion' ( 12 ) , 293T (epithelial) ( 7 ) , B lymphocyte-spleen ( 19 ) , BMMC (mast) ( 2 ) , bone marrow ( 18 ) , colon ( 1 , 8 , 16 ) , liver ( 11 , 13 ) , macrophage ( 9 ) , mast-bone marrow [SHP-2 (mouse), homozygous knockout] ( 10 ) , MDA-MB-453 (breast cell) ( 11 ) , MEF (fibroblast) ( 14 ) , microvessel endothelial-lung ( 5 ) , monocyte ( 9 ) , myocyte-heart ( 17 ) , osteoclast ( 9 ) , RAW 264.7 (macrophage) ( 6 , 15 ) , spleen ( 18 )

Upstream Regulation
Regulatory protein:
BRAF (human) ( 12 ) , CAPN1 (mouse) ( 2 ) , ILK (mouse) ( 1 ) , SHP-2 (mouse) ( 10 ) , TSC2 (mouse) ( 14 )
Putative in vivo kinases:
AMPKA2 (mouse) ( 5 ) , IKKB (mouse) ( 7 )
Treatments:
anti-CD40 ( 18 , 19 ) , anti-IgE ( 10 ) , anti-IgM ( 19 ) , bacterial infection ( 8 , 16 ) , bortezomib ( 16 ) , cAMP_analog ( 15 ) , GDNF ( 12 ) , GSK-3_inhibitor_X ( 6 ) , high-fat diet ( 11 ) , IFN-gamma ( 6 ) , IL-10 ( 18 ) , KT5720 ( 15 ) , LPS ( 17 , 18 ) , MMS ( 14 ) , NGF ( 12 ) , NS398 ( 15 ) , NSC-87877 ( 6 ) , partial_hepatectomy ( 13 ) , PD98059 ( 8 ) , peptidoglycan ( 15 ) , PGE2 ( 15 ) , RANKL ( 9 ) , siRNA ( 12 ) , TNF ( 7 , 9 , 14 ) , U0126 ( 14 ) , wedelolactone ( 7 )

Downstream Regulation
Effects of modification on IKKB:
enzymatic activity, induced ( 9 ) , molecular association, regulation ( 7 ) , phosphorylation ( 7 )
Effects of modification on biological processes:
apoptosis, altered ( 12 ) , cell differentiation, induced ( 9 )
Induce interaction with:
PPP2R3C (human) ( 7 )

References 

1

Ahmed AU, et al. (2017) Integrin-Linked Kinase Expression in Myeloid Cells Promotes Inflammatory Signaling during Experimental Colitis. J Immunol
28794235   Curated Info

2

Wu Z, et al. (2014) Calpain-1 contributes to IgE-mediated mast cell activation. J Immunol 192, 5130-9
24760147   Curated Info

3

Yan J, et al. (2013) Inactivation of BAD by IKK inhibits TNFα-induced apoptosis independently of NF-κB activation. Cell 152, 304-15
23332762   Curated Info

4

Sarkar S, et al. (2011) Complex inhibitory effects of nitric oxide on autophagy. Mol Cell 43, 19-32
21726807   Curated Info

5

Bess E, Fisslthaler B, Frömel T, Fleming I (2011) Nitric oxide-induced activation of the AMP-activated protein kinase α2 subunit attenuates IκB kinase activity and inflammatory responses in endothelial cells. PLoS One 6, e20848
21673972   Curated Info

6

Kai JI, et al. (2010) Glycogen synthase kinase-3β indirectly facilitates interferon-γ-induced nuclear factor-κB activation and nitric oxide biosynthesis. J Cell Biochem 111, 1522-30
20872791   Curated Info

7

Chiang CW, Liu WK, Chiang CW, Chou CK (2010) Phosphorylation-dependent association of the G4-1/G5PR regulatory subunit with IKKβ negatively modulates NF-κB activation through recruitment of protein phosphatase 5. Biochem J 433, 187-96
20925653   Curated Info

8

Chandrakesan P, et al. (2010) Novel changes in NF-{kappa}B activity during progression and regression phases of hyperplasia: role of MEK, ERK, and p38. J Biol Chem 285, 33485-98
20710027   Curated Info

9

Darwech I, Otero JE, Alhawagri MA, Abu-Amer Y (2010) Tyrosine phosphorylation is required for IkappaB kinase-beta (IKKbeta) activation and function in osteoclastogenesis. J Biol Chem 285, 25522-30
20534585   Curated Info

10

McPherson VA, et al. (2009) SH2 domain-containing phosphatase-2 protein-tyrosine phosphatase promotes Fc(epsilon)RI-induced activation of Fyn and Erk pathways leading to TNF(alpha) release from bone marrow-derived mast cells. J Immunol 183, 4940-7
19786542   Curated Info

11

Lee DF, et al. (2008) IKKbeta suppression of TSC1 function links the mTOR pathway with insulin resistance. Int J Mol Med 22, 633-8
18949383   Curated Info

12

Encinas M, et al. (2008) Analysis of Ret knockin mice reveals a critical role for IKKs, but not PI 3-K, in neurotrophic factor-induced survival of sympathetic neurons. Cell Death Differ 15, 1510-21
18497757   Curated Info

13

Malato Y, et al. (2008) Hepatocyte-specific inhibitor-of-kappaB-kinase deletion triggers the innate immune response and promotes earlier cell proliferation during liver regeneration. Hepatology 47, 2036-50
18393321   Curated Info

14

Ghosh S, et al. (2006) Essential role of tuberous sclerosis genes TSC1 and TSC2 in NF-kappaB activation and cell survival. Cancer Cell 10, 215-26
16959613   Curated Info

15

Chen BC, et al. (2006) Peptidoglycan-induced IL-6 production in RAW 264.7 macrophages is mediated by cyclooxygenase-2, PGE2/PGE4 receptors, protein kinase A, I kappa B kinase, and NF-kappa B. J Immunol 177, 681-93
16785567   Curated Info

16

Wang Y, Xiang GS, Kourouma F, Umar S (2006) Citrobacter rodentium-induced NF-kappaB activation in hyperproliferating colonic epithelia: role of p65 (Ser536) phosphorylation. Br J Pharmacol 148, 814-24
16751795   Curated Info

17

Hall G, et al. (2005) Inhibitor-kappaB kinase-beta regulates LPS-induced TNF-alpha production in cardiac myocytes through modulation of NF-kappaB p65 subunit phosphorylation. Am J Physiol Heart Circ Physiol 289, H2103-11
15980040   Curated Info

18

Bhattacharyya S, et al. (2004) Immunoregulation of dendritic cells by IL-10 is mediated through suppression of the PI3K/Akt pathway and of IkappaB kinase activity. Blood 104, 1100-9
15113757   Curated Info

19

Saijo K, et al. (2002) Protein kinase C beta controls nuclear factor kappaB activation in B cells through selective regulation of the IkappaB kinase alpha. J Exp Med 195, 1647-52
12070292   Curated Info