Ser120
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Home > Phosphorylation Site Page: > Ser120  -  NME1 (human)

Site Information
GRNIIhGsDsVEsAE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450098

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 14 ) , immunoprecipitation ( 15 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 7 , 9 , 10 , 11 ) , mass spectrometry (in vitro) ( 12 ) , mutation of modification site ( 14 , 15 ) , western blotting ( 15 )
Disease tissue studied:
breast cancer ( 9 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 ) , lung cancer ( 4 , 9 ) , non-small cell lung cancer ( 9 ) , non-small cell lung adenocarcinoma ( 4 ) , prostate cancer ( 14 ) , melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:
breast ( 2 ) , BT-20 (breast cell) ( 9 ) , BT-549 (breast cell) ( 9 ) , COS (fibroblast) ( 15 ) , DU 145 (prostate cell) ( 14 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 10 ) , Flp-In T-Rex-293 (epithelial) ( 10 ) , H2009 (pulmonary) ( 9 ) , H2077 (pulmonary) ( 9 ) , H2887 (pulmonary) ( 9 ) , H322M (pulmonary) ( 9 ) , HCC1359 (pulmonary) ( 9 ) , HCC1937 (breast cell) ( 9 ) , HCC2279 (pulmonary) ( 9 ) , HCC366 (pulmonary) ( 9 ) , HCC4006 (pulmonary) ( 9 ) , HCC78 (pulmonary) ( 9 ) , HeLa (cervical) ( 1 , 5 , 7 ) , HOP62 (pulmonary) ( 9 ) , K562 (erythroid) ( 7 , 11 ) , MCF-7 (breast cell) ( 9 ) , MDA-MB-231 (breast cell) ( 9 ) , MDA-MB-468 (breast cell) ( 9 ) , NCI-H1395 (pulmonary) ( 9 ) , NCI-H1568 (pulmonary) ( 9 ) , NCI-H157 (pulmonary) ( 9 ) , NCI-H1666 (pulmonary) ( 9 ) , NCI-H2030 (pulmonary) ( 9 ) , NCI-H2172 (pulmonary) ( 9 ) , NCI-H322 (pulmonary) ( 9 ) , NCI-H520 (squamous) ( 9 ) , NCI-H647 (pulmonary) ( 9 ) , PC9 (pulmonary) ( 4 , 9 ) , WM239A (melanocyte) ( 3 )

Upstream Regulation
Kinases, in vitro:
CDK1 (human) ( 12 ) , NME1 (human) ( 13 , 16 )
Treatments:
dasatinib ( 11 )

Downstream Regulation
Effects of modification on NME1:
enzymatic activity, induced ( 14 , 16 ) , molecular association, regulation ( 15 ) , protein conformation ( 13 , 14 )
Effects of modification on biological processes:
cell growth, altered ( 14 )
Induce interaction with:
PRUNE (human) ( 15 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

4

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

7

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

8

King JD, et al. (2012) Role of Binding and Nucleoside Diphosphate Kinase A in the Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator by AMP-activated Protein Kinase. J Biol Chem 287, 33389-400
22869372   Curated Info

9

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

10

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

11

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

12

Blethrow JD, Glavy JS, Morgan DO, Shokat KM (2008) Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates. Proc Natl Acad Sci U S A 105, 1442-7
18234856   Curated Info

13

Mocan I, et al. (2007) Protein phosphorylation corrects the folding defect of the neuroblastoma (S120G) mutant of human nucleoside diphosphate kinase A/Nm23-H1. Biochem J 403, 149-56
17155928   Curated Info

14

Kim YI, Park S, Jeoung DI, Lee H (2003) Point mutations affecting the oligomeric structure of Nm23-H1 abrogates its inhibitory activity on colonization and invasion of prostate cancer cells. Biochem Biophys Res Commun 307, 281-9
12859952   Curated Info

15

Reymond A, et al. (1999) Evidence for interaction between human PRUNE and nm23-H1 NDPKinase. Oncogene 18, 7244-52
10602478   Curated Info

16

Freije JM, et al. (1997) Site-directed mutation of Nm23-H1. Mutations lacking motility suppressive capacity upon transfection are deficient in histidine-dependent protein phosphotransferase pathways in vitro. J Biol Chem 272, 5525-32
9038158   Curated Info

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