Ser44
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Home > Phosphorylation Site Page: > Ser44  -  NME1 (human)

Site Information
GLkFMQAsEDLLkEH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450096

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 3 , 4 ) , western blotting ( 4 )
Disease tissue studied:
prostate cancer ( 3 ) , ventricular tachycardia ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
NME1 (human) ( 6 )
Treatments:
colforsin ( 6 )

Downstream Regulation
Effects of modification on biological processes:
cell growth, altered ( 3 )

References 

1

Li Y (2011) CST Curation Set: 10519; Year: 2011; Biosample/Treatment: tissue, heart/untreated; Disease: ventricular tachycardia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

3

Kim YI, Park S, Jeoung DI, Lee H (2003) Point mutations affecting the oligomeric structure of Nm23-H1 abrogates its inhibitory activity on colonization and invasion of prostate cancer cells. Biochem Biophys Res Commun 307, 281-9
12859952   Curated Info

4

Reymond A, et al. (1999) Evidence for interaction between human PRUNE and nm23-H1 NDPKinase. Oncogene 18, 7244-52
10602478   Curated Info

5

Freije JM, et al. (1997) Site-directed mutation of Nm23-H1. Mutations lacking motility suppressive capacity upon transfection are deficient in histidine-dependent protein phosphotransferase pathways in vitro. J Biol Chem 272, 5525-32
9038158   Curated Info

6

MacDonald NJ, et al. (1993) A serine phosphorylation of Nm23, and not its nucleoside diphosphate kinase activity, correlates with suppression of tumor metastatic potential. J Biol Chem 268, 25780-9
8245015   Curated Info