Thr686
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr686  -  TTK (human)

Site Information
VkDSQVGtVNYMPPE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 487541

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 9 ) , electrophoretic mobility shift ( 8 ) , immunoprecipitation ( 1 , 2 , 4 , 6 ) , mass spectrometry ( 1 , 2 , 8 , 9 ) , mass spectrometry (in vitro) ( 7 ) , mutation of modification site ( 1 , 2 , 3 , 5 , 6 , 8 , 9 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 , 9 ) , western blotting ( 1 , 2 , 4 , 5 , 9 )
Disease tissue studied:
bone cancer ( 1 , 8 , 9 ) , cervical cancer ( 1 ) , cervical adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
TTK (human) ( 1 , 4 , 5 )
Kinases, in vitro:
TTK (human) ( 5 , 6 , 7 , 8 , 9 )
Treatments:
AP20187 ( 1 ) , okadaic_acid ( 2 )

Downstream Regulation
Effects of modification on TTK:
enzymatic activity, induced ( 5 , 6 , 9 )
Effects of modification on biological processes:
cell cycle regulation ( 8 )

References 

1

Combes G, et al. (2018) Mps1 Phosphorylates Its N-Terminal Extension to Relieve Autoinhibition and Activate the Spindle Assembly Checkpoint. Curr Biol 28, 872-883.e5
29502948   Curated Info

2

Diril MK, et al. (2016) Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet 12, e1006310
27631493   Curated Info

3

Wang X, et al. (2014) Dynamic autophosphorylation of mps1 kinase is required for faithful mitotic progression. PLoS One 9, e104723
25265012   Curated Info

4

Lee S, et al. (2012) Characterization of spindle checkpoint kinase Mps1 reveals domain with functional and structural similarities to tetratricopeptide repeat motifs of Bub1 and BubR1 checkpoint kinases. J Biol Chem 287, 5988-6001
22187426   Curated Info

5

Wang W, et al. (2009) Structural and mechanistic insights into Mps1 kinase activation. J Cell Mol Med 13, 1679-94
19120698   Curated Info

6

Tyler RK, et al. (2009) Phosphoregulation of human Mps1 kinase. Biochem J 417, 173-81
18680479   Curated Info

7

Xu Q, et al. (2009) Regulation of kinetochore recruitment of two essential mitotic spindle checkpoint proteins by Mps1 phosphorylation. Mol Biol Cell 20, 10-20
18923149   Curated Info

8

Jelluma N, et al. (2008) Chromosomal instability by inefficient Mps1 auto-activation due to a weakened mitotic checkpoint and lagging chromosomes. PLoS One 3, e2415
18545697   Curated Info

9

Mattison CP, et al. (2007) Mps1 activation loop autophosphorylation enhances kinase activity. J Biol Chem 282, 30553-61
17728254   Curated Info