Thr676
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Home > Phosphorylation Site Page: > Thr676  -  TTK (human)

Site Information
NQMQPDttsVVkDSQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 487540

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 12 ) , [32P] ATP in vitro ( 1 , 13 ) , electrophoretic mobility shift ( 1 , 11 ) , immunoassay ( 1 , 5 ) , immunoprecipitation ( 2 , 3 , 8 , 10 ) , mass spectrometry ( 2 , 3 , 11 , 12 , 13 ) , mutation of modification site ( 1 , 2 , 3 , 7 , 9 , 10 , 11 , 12 , 13 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 10 , 11 , 13 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 8 , 9 , 11 , 13 )
Disease tissue studied:
bone cancer ( 2 , 6 , 11 , 13 ) , cervical cancer ( 2 ) , cervical adenocarcinoma ( 2 ) , colorectal cancer ( 4 ) , colorectal carcinoma ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PLK1 (human) ( 5 ) , TTK (human) ( 1 , 2 , 5 , 8 )
Kinases, in vitro:
PLK1 (human) ( 5 ) , TTK (human) ( 1 , 9 , 10 , 11 , 12 , 13 , 14 )
Putative upstream phosphatases:
PPP2R5D (human) ( 1 )
Treatments:
AP20187 ( 12 ) , AZ3146 ( 1 ) , BI2536 ( 5 ) , calyculin_A ( 1 ) , CCT251455 ( 4 ) , nocodazole ( 11 , 12 ) , okadaic_acid ( 3 ) , reversine ( 5 ) , siRNA ( 1 ) , tautomycetin ( 1 ) , ZK-Thiazolidinone ( 5 )

Downstream Regulation
Effects of modification on TTK:
activity, induced ( 1 ) , enzymatic activity, induced ( 5 , 10 , 11 , 12 , 13 ) , intracellular localization ( 5 ) , molecular association, regulation ( 12 )
Effects of modification on biological processes:
cell cycle regulation ( 1 , 11 , 12 ) , chromatin organization, altered ( 1 )

References 

1

Hayward D, et al. (2019) Checkpoint signaling and error correction require regulation of the MPS1 T-loop by PP2A-B56. J Cell Biol 218, 3188-3199
31511308   Curated Info

2

Combes G, et al. (2018) Mps1 Phosphorylates Its N-Terminal Extension to Relieve Autoinhibition and Activate the Spindle Assembly Checkpoint. Curr Biol 28, 872-883.e5
29502948   Curated Info

3

Diril MK, et al. (2016) Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet 12, e1006310
27631493   Curated Info

4

Gurden MD, et al. (2015) Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance. Cancer Res 75, 3340-54
26202014   Curated Info

5

von Schubert C, et al. (2015) Plk1 and Mps1 Cooperatively Regulate the Spindle Assembly Checkpoint in Human Cells. Cell Rep 12, 66-78
26119734   Curated Info

6

Nijenhuis W, et al. (2014) Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol 16, 1257-64
25402682   Curated Info

7

Wang X, et al. (2014) Dynamic autophosphorylation of mps1 kinase is required for faithful mitotic progression. PLoS One 9, e104723
25265012   Curated Info

8

Lee S, et al. (2012) Characterization of spindle checkpoint kinase Mps1 reveals domain with functional and structural similarities to tetratricopeptide repeat motifs of Bub1 and BubR1 checkpoint kinases. J Biol Chem 287, 5988-6001
22187426   Curated Info

9

Wang W, et al. (2009) Structural and mechanistic insights into Mps1 kinase activation. J Cell Mol Med 13, 1679-94
19120698   Curated Info

10

Tyler RK, et al. (2009) Phosphoregulation of human Mps1 kinase. Biochem J 417, 173-81
18680479   Curated Info

11

Jelluma N, et al. (2008) Chromosomal instability by inefficient Mps1 auto-activation due to a weakened mitotic checkpoint and lagging chromosomes. PLoS One 3, e2415
18545697   Curated Info

12

Kang J, Chen Y, Zhao Y, Yu H (2007) Autophosphorylation-dependent activation of human Mps1 is required for the spindle checkpoint. Proc Natl Acad Sci U S A 104, 20232-7
18083840   Curated Info

13

Mattison CP, et al. (2007) Mps1 activation loop autophosphorylation enhances kinase activity. J Biol Chem 282, 30553-61
17728254   Curated Info

14

Lindberg RA, Fischer WH, Hunter T (1993) Characterization of a human protein threonine kinase isolated by screening an expression library with antibodies to phosphotyrosine. Oncogene 8, 351-9
7678926   Curated Info