Ser381
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Home > Phosphorylation Site Page: > Ser381  -  TNFAIP3 (human)

Site Information
EPsVPQLsLMDVKCE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 487535

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 9 ) , immunoprecipitation ( 9 ) , mass spectrometry ( 2 , 3 , 5 , 6 , 8 , 9 ) , mass spectrometry (in vitro) ( 4 ) , mutation of modification site ( 1 , 2 , 4 ) , phospho-antibody ( 2 , 9 ) , western blotting ( 2 , 9 )
Disease tissue studied:
HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 ) , pancreatic cancer ( 2 ) , pancreatic carcinoma ( 2 ) , melanoma skin cancer ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 , 9 ) , beta TC3 (pancreatic) ( 2 ) , breast ( 3 ) , E.coli (bacterial) ( 4 ) , HeLa (cervical) ( 8 ) , Jurkat (T lymphocyte) ( 6 ) , monocyte-blood ( 2 ) , T lymphocyte ( 2 ) , WM239A (melanocyte) ( 5 )

Upstream Regulation
Putative in vivo kinases:
IKKB (human) ( 2 , 9 )
Kinases, in vitro:
IKKB (human) ( 9 )
Treatments:
BMS-345541 ( 9 ) , TNF ( 2 )

Downstream Regulation
Effects of modification on TNFAIP3:
activity, induced ( 1 , 2 , 9 ) , ubiquitination ( 2 , 4 )
Effects of modification on biological processes:
signaling pathway regulation ( 2 , 9 ) , transcription, induced ( 2 )

References 

1

Cultrone D, et al. (2020) A zebrafish functional genomics model to investigate the role of human A20 variants in vivo. Sci Rep 10, 19085
33154446   Curated Info

2

Zammit NW, et al. (2019) Denisovan, modern human and mouse TNFAIP3 alleles tune A20 phosphorylation and immunity. Nat Immunol 20, 1299-1310
31534238   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Wertz IE, et al. (2015) Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation. Nature 528, 370-5
26649818   Curated Info

5

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

6

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

7

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

8

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

9

Hutti JE, et al. (2007) I{kappa}B Kinase {beta} Phosphorylates the K63 Deubiquitinase A20 To Cause Feedback Inhibition of the NF-{kappa}B Pathway. Mol Cell Biol 27, 7451-61
17709380   Curated Info